| Title: | "Backbone and side chain NMR assignments and secondary structure calculation of the pheromone binding protein3 of Ostrinia nubilalis, an agricultural pest" |
| Address: | "Department of Chemistry, Oklahoma State University, Stillwater, OK, 74078, USA. Department of Chemistry, Oklahoma State University, Stillwater, OK, 74078, USA. smita.mohanty@okstate.edu" |
| DOI: | 10.1007/s12104-023-10145-3 |
| ISSN/ISBN: | 1874-270X (Electronic) 1874-270X (Linking) |
| Abstract: | "Ostrinia nubilalis, also known as European Corn Borer (ECB), is a serious pest in Europe and North America, as well as in Central Asia and Northern Africa. It damages a variety of agricultural crops such as corn, oats, buckwheat, millet, and soybeans. causing annually at least one billion dollars in loss. The Ostrinia nubilalis pheromone-binding protein3 (OnubPBP3), preferentially expressed in the male moth antenna, has been implicated in the detection of the female-secreted pheromone blend during the mating process. Understanding the structure of and function of OnubPBP3, including the mechanism of pheromone binding and its release at the dendritic olfactory neuron (ORN), is essential if integrated pest management through sensory inhibition is to be achieved. We report here the backbone and side-chain resonance assignments of OnubPBP3 at pH 6.5 using various triple resonance NMR experiments on a (13)C, (15)N-labeled protein sample. The secondary structure of OnubPBP3 consists of six alpha-helices and an unstructured C-terminus based on backbone chemical shifts" |
| Keywords: | Agricultural pest NMR assignment Ostrinia nubilalis Pheromone binding proteins (PBPs); |
| Notes: | "PublisherAl-Danoon, Omar Mohanty, Smita eng CHE-1807722/National Science Foundation/ DBI-1726397/National Science Foundation, / Netherlands 2023/07/27 Biomol NMR Assign. 2023 Jul 27. doi: 10.1007/s12104-023-10145-3" |
