| Title: | The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles |
| Author(s): | Terry MI; Perez-Sanz F; Diaz-Galian MV; Perez de Los Cobos F; Navarro PJ; Egea-Cortines M; Weiss J; |
| Address: | "Genetica Molecular, Instituto de Biotecnologia Vegetal, Edificio I+D+I, Plaza del Hospital s/n, Universidad Politecnica de Cartagena, 30202 Cartagena, Spain. marta.terry@edu.upct.es. Biomedical Informatic and Bioinformatic Platform, Biomedical Research Institute of Murcia, University Clinical Hospital 'Virgen de la Arrixaca', University of Murcia, 30120 Murcia, Spain. fernando.perez8@um.es. Genetica Molecular, Instituto de Biotecnologia Vegetal, Edificio I+D+I, Plaza del Hospital s/n, Universidad Politecnica de Cartagena, 30202 Cartagena, Spain. mariavictoria.diaz@edu.upct.es. Plant Breeding Department, Center of Edafology and Applied Biology of Segura-High Council for Scientific Research (CEBAS-CSIC), Espinardo University Campus, Espinardo, 30100 Murcia, Spain. fpcobos@cebas.csic.es. Escuela Tecnica Superior de Ingenieria de Telecomunicacion (DSIE), Campus Muralla del Mar, s/n., Universidad Politecnica de Cartagena, 30202 Cartagena, Spain. pedroj.navarro@upct.es. Genetica Molecular, Instituto de Biotecnologia Vegetal, Edificio I+D+I, Plaza del Hospital s/n, Universidad Politecnica de Cartagena, 30202 Cartagena, Spain. Marcos.Egea@upct.es. Genetica Molecular, Instituto de Biotecnologia Vegetal, Edificio I+D+I, Plaza del Hospital s/n, Universidad Politecnica de Cartagena, 30202 Cartagena, Spain. Julia.weiss@upct.es" |
| ISSN/ISBN: | 2073-4409 (Print) 2073-4409 (Electronic) 2073-4409 (Linking) |
| Abstract: | "The floral perianth, comprising sepals and petals, conceals the sexual organs and attracts pollinators. The coordination of growth and scent emission is not fully understood. We have analyzed the effect of knocking down CHANEL (PhCHL), the ZEITLUPE ortholog in petunia (PhCHL) by hairpin RNAs. Plants with low PhCHL mRNA had overall decreased size. Growth evaluation using time lapse image analysis showed that early leaf movement was not affected by RNAi:PhCHL, but flower angle movement was modified, moving earlier during the day in knockdown plants than in wild types. Despite differences in stem length, growth rate was not significantly affected by loss of PhCHL. In contrast, petal growth displayed lower growth rate in RNAi:PhCHL. Decreased levels of PhCHL caused strongly modified scent profiles, including changes in composition and timing of emission resulting in volatile profiles highly divergent from the wild type. Our results show a role of PhCHL in controlling growth and development of vegetative and reproductive organs in petunia. The different effects of PhCHL on organ development indicate an organ-specific interpretation of the down regulation of PhCHL. Through the control of both timing and quantitative volatile emissions, PhCHL appears to be a major coordinator of scent profiles" |
| Keywords: | "Flowers/*growth & development Gene Expression Regulation, Plant Odorants/*analysis *Period Circadian Proteins/genetics/physiology *Petunia/genetics/growth & development Zeitlupe circadian clock floral scent growth rate hairpin RNA image analysis petal dev;" |
| Notes: | "MedlineTerry, Marta I Perez-Sanz, Fernando Diaz-Galian, M Victoria Perez de Los Cobos, Felipe Navarro, Pedro J Egea-Cortines, Marcos Weiss, Julia eng Research Support, Non-U.S. Gov't Switzerland 2019/04/14 Cells. 2019 Apr 11; 8(4):343. doi: 10.3390/cells8040343" |
