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Bioresour Technol

Title:		Use of mixed culture bacteria for photofermentive hydrogen of dark fermentation effluent
Author(s):		Tawfik A; El-Bery H; Kumari S; Bux F;
Address:		"Egypt-Japan University of Science and Technology (E-Just), Environmental Engineering Department, P.O. Box 179, New Borg El Arab City, 21934 Alexandria, Egypt. Institute for Water and Wastewater Technology, Durban University of Technology, P.O. Box 1334, Durban 4000, South Africa. Institute for Water and Wastewater Technology, Durban University of Technology, P.O. Box 1334, Durban 4000, South Africa. Electronic address: faizalb@dut.ac.za"
Journal Title:		Bioresour Technol
Year:		2014
Volume:		20140326
Issue:
Page Number:		119 - 126
DOI:		10.1016/j.biortech.2014.03.065
ISSN/ISBN:		1873-2976 (Electronic) 0960-8524 (Linking)
Abstract:		"Hydrogen production (HP) from dark fermentation effluent of starch wastewater via vertical tubular photo-bioreactor was investigated. The reactor was inoculated with mixed culture of bacteria and operated at light intensity of 190 W/m(2). Hydraulic retention time (HRT) and organic loading rate (OLR) was varied between 0.9 to 4.0 h and 3.2 to 16 g COD/l.d., respectively. Increasing the HRT from 0.9 to 2.5 h, significantly (P<0.05) increased HP from 1+/-0.04 to 3.05+/-0.19 l/d, respectively. However, minimal increase in HP occurred when increasing the HRT up to 4.0 h. The HP remained unaffected when increasing the OLR from 3.2 to 6.4 g COD/l.d. Further increase in the OLR up to 8.2 and 16 g COD/l.d., resulted in a drop in HP i.e. 0.96 and 0.19 l/d, respectively. Microbial community analysis of the reactor samples showed the presence and dominance of hydrogen producing purple non-sulfur phototrophic (PNS) bacterium, Rhodopseudomonas palustris in the reactor"
Keywords:		"Ammonium Compounds/analysis Bacteria/*metabolism Biological Oxygen Demand Analysis Carboxylic Acids/analysis *Darkness Denaturing Gradient Gel Electrophoresis Fatty Acids, Volatile/analysis *Fermentation Hydrogen/*metabolism Photobioreactors/microbiology;"
Notes:		"MedlineTawfik, Ahmed El-Bery, Haitham Kumari, Sheena Bux, Faizal eng Research Support, Non-U.S. Gov't England 2014/04/29 Bioresour Technol. 2014 Sep; 168:119-26. doi: 10.1016/j.biortech.2014.03.065. Epub 2014 Mar 26"