« Previous AbstractSynthetic morphology using alternative inputs    Next AbstractA pcl-like cyclin activates the Res2p-Cdc10p cell cycle 'start' transcriptional factor complex in fission yeast »

Mol Cell Biol

Title:		Schizosaccharomyces pombe map3+ encodes the putative M-factor receptor
Author(s):		Tanaka K; Davey J; Imai Y; Yamamoto M;
Address:		"Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan"
Journal Title:		Mol Cell Biol
Year:		1993
Volume:		13
Issue:		1
Page Number:		80 - 88
DOI:		10.1128/mcb.13.1.80-88.1993
ISSN/ISBN:		0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking)
Abstract:		"A defect in the map3 gene of the fission yeast Schizosaccharomyces pombe causes h+ mating-type-specific sterility. This gene was cloned by complementation. Nucleotide sequence analysis showed that it has a coding capacity of 365 amino acids. The deduced map3 gene product is a putative seven-transmembrane protein and has 20.0% amino acid identity with the a-factor receptor of Saccharomyces cerevisiae, encoded by STE3. It is also homologous with the Ustilago maydis mating pheromone receptors. The map3 gene is expressed in h+ cells but not in h- cells, and the transcripts are induced in response to nitrogen starvation. h+ cells defective in map3 do not respond to purified M-factor. When map3 is expressed ectopically in h- cells, they apparently acquire the ability to respond to the M-factor produced by themselves. The gpa1 gene, which encodes the alpha-subunit of a G-protein presumed to couple with the mating pheromone receptors, is essential for this function of map3. These observations strongly suggest that map3 encodes the M-factor receptor. Furthermore, this study provides strong support for the notion that pheromone signaling is essential for initiation of meiosis in S. pombe and that either M-factor signaling or P-factor signaling alone is sufficient"
Keywords:		"Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Fungal/genetics Gene Expression *Genes, Fungal Genetic Complementation Test Meiosis Molecular Sequence Data Mutagenesis RNA, Fungal/genetics RNA, Messenger/genetics Receptors, Cell Surface/chemistr;"
Notes:		"MedlineTanaka, K Davey, J Imai, Y Yamamoto, M eng Comparative Study Research Support, Non-U.S. Gov't 1993/01/01 Mol Cell Biol. 1993 Jan; 13(1):80-8. doi: 10.1128/mcb.13.1.80-88.1993"