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Front Cell Neurosci

Title:		Putative Neural Network Within an Olfactory Sensory Unit for Nestmate and Non-nestmate Discrimination in the Japanese Carpenter Ant: The Ultra-structures and Mathematical Simulation
Author(s):		Takeichi Y; Uebi T; Miyazaki N; Murata K; Yasuyama K; Inoue K; Suzaki T; Kubo H; Kajimura N; Takano J; Omori T; Yoshimura R; Endo Y; Hojo MK; Takaya E; Kurihara S; Tatsuta K; Ozaki K; Ozaki M;
Address:		"Department of Biology, Graduate School of Science, Kobe University, Kobe, Japan. National Institute for Physiological Sciences, Okazaki, Japan. Division of Biology, Department of Natural Sciences, Kawasaki Medical School, Kurashiki, Japan. Research Center for Ultra-High Voltage Electron Microscopy, Osaka University, Ibaraki, Japan. Department of Mathematics, Faculty of Sciences, Hokkaido University, Sapporo, Japan. Graduate School of Frontier Biosciences, Osaka University, Suita, Japan. Department of Electrical and Electronic Engineering, Graduate School of Engineering, Kobe University, Kobe, Japan. Department of Applied Biology, Kyoto Institute of Technology, Kyoto, Japan. Graduate School of Information Systems, The University of Electro-Communications, Chofu, Japan. Department of Biological Science, Faculty of Life and Environmental Science, Shimane University, Matsue, Japan"
Journal Title:		Front Cell Neurosci
Year:		2018
Volume:		20180919
Issue:
Page Number:		310 -
DOI:		10.3389/fncel.2018.00310
ISSN/ISBN:		1662-5102 (Print) 1662-5102 (Electronic) 1662-5102 (Linking)
Abstract:		"Ants are known to use a colony-specific blend of cuticular hydrocarbons (CHCs) as a pheromone to discriminate between nestmates and non-nestmates and the CHCs were sensed in the basiconic type of antennal sensilla (S. basiconica). To investigate the functional design of this type of antennal sensilla, we observed the ultra-structures at 2D and 3D in the Japanese carpenter ant, Camponotus japonicus, using a serial block-face scanning electron microscope (SBF-SEM), and conventional and high-voltage transmission electron microscopes. Based on the serial images of 352 cross sections of SBF-SEM, we reconstructed a 3D model of the sensillum revealing that each S. basiconica houses > 100 unbranched dendritic processes, which extend from the same number of olfactory receptor neurons (ORNs). The dendritic processes had characteristic beaded-structures and formed a twisted bundle within the sensillum. At the 'beads,' the cell membranes of the processes were closely adjacent in the interdigitated profiles, suggesting functional interactions via gap junctions (GJs). Immunohistochemistry with anti-innexin (invertebrate GJ protein) antisera revealed positive labeling in the antennae of C. japonicus. Innexin 3, one of the five antennal innexin subtypes, was detected as a dotted signal within the S. basiconica as a sensory organ for nestmate recognition. These morphological results suggest that ORNs form an electrical network via GJs between dendritic processes. We were unable to functionally certify the electric connections in an olfactory sensory unit comprising such multiple ORNs; however, with the aid of simulation of a mathematical model, we examined the putative function of this novel chemosensory information network, which possibly contributes to the distinct discrimination of colony-specific blends of CHCs or other odor detection"
Keywords:		ant chemical communication chemosensillum innexin mathematical simulation olfactory receptor ultra-structures;
Notes:		"PubMed-not-MEDLINETakeichi, Yusuke Uebi, Tatsuya Miyazaki, Naoyuki Murata, Kazuyoshi Yasuyama, Kouji Inoue, Kanako Suzaki, Toshinobu Kubo, Hideo Kajimura, Naoko Takano, Jo Omori, Toshiaki Yoshimura, Ryoichi Endo, Yasuhisa Hojo, Masaru K Takaya, Eichi Kurihara, Satoshi Tatsuta, Kenta Ozaki, Koichi Ozaki, Mamiko eng Switzerland 2018/10/05 Front Cell Neurosci. 2018 Sep 19; 12:310. doi: 10.3389/fncel.2018.00310. eCollection 2018"