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Insect Mol Biol

Title:		The terpenoid backbone biosynthesis pathway directly affects the biosynthesis of alarm pheromone in the aphid
Author(s):		Sun ZJ; Li ZX;
Address:		"Department of Entomology, MOA Key Laboratory for Monitoring and Environment-Friendly Control of Crop Pests, College of Plant Protection, China Agricultural University, Beijing, China"
Journal Title:		Insect Mol Biol
Year:		2018
Volume:		20180912
Issue:		6
Page Number:		824 - 834
DOI:		10.1111/imb.12521
ISSN/ISBN:		1365-2583 (Electronic) 0962-1075 (Linking)
Abstract:		"The terpenoid backbone biosynthesis pathway is responsible for the synthesis of different backbones for terpenoids; (E)-beta-farnesene (EbetaF), a sesquiterpene, is the major component of aphid alarm pheromone. Our previous studies eliminated the possibility of host plants and endosymbionts as the sources of EbetaF, and we thus speculate that the terpenoid pathway might affect the biosynthesis of EbetaF in aphids. First, the transcriptional responses of four genes encoding farnesyl diphosphate synthase (FPPS), geranylgeranyl diphosphate synthase (GGPPS) and decaprenyl diphosphate synthase in the cotton aphid Aphis gossypii to simulated stimulation were analysed using quantitative real-time PCR, showing an immediate decrease in the transcript abundances of the four genes. Next, RNA-interference-mediated gene knockdown was performed, indicating that fpps knockdown caused a significant cost in terms of body size and fecundity. Finally, an association analysis of gene knockdown with the amount of EbetaF was conducted, revealing that the concentration of EbetaF per milligram of aphid was drastically decreased in response to fpps knockdown, whereas ggpps knockdown significantly raised the concentration of EbetaF. Our data support a peculiar mode of biosynthesis and storage of the aphid alarm pheromone that relies directly on the terpenoid backbone biosynthesis pathway in the aphid"
Keywords:		Animals Aphids/genetics/*metabolism Body Size Fertility Gene Expression Regulation Mortality Pheromones/*biosynthesis RNA Interference Sesquiterpenes/*metabolism (E)-beta-farnesene Aphis gossypii.RNAi decaprenyl diphosphate synthase farnesyl diphosphate;
Notes:		"MedlineSun, Z-J Li, Z-X eng Research Support, Non-U.S. Gov't England 2018/07/25 Insect Mol Biol. 2018 Dec; 27(6):824-834. doi: 10.1111/imb.12521. Epub 2018 Sep 12"