| Title: | Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Brauchler: Effect of Carbon Source |
| Author(s): | Stojicic D; Tosic S; Stojanovic G; Zlatkovic B; Jovanovic S; Budimir S; Uzelac B; |
| Address: | "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Nis, Visegradska 33, 18000 Niche, Serbia. Department of Chemistry, Faculty of Sciences and Mathematics, University of Nis, Visegradska 33, 18000 Niche, Serbia. Department of Plant Physiology, Institute for Biological Research 'Sinisa Stankovic', National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia" |
| ISSN/ISBN: | 2223-7747 (Print) 2223-7747 (Electronic) 2223-7747 (Linking) |
| Abstract: | "Clinopodium pulegium (Rochel) Brauchler (Lamiaceae) is an endangered species endemic to the Southern Carpathians. It is characterized by the production of high amounts of essential oils, which emit volatile organic compounds (VOCs) that have an essential role in biotic and abiotic stress responses and in plant-plant and plant-insect interactions. The present study was initiated to phytochemically examine the influence of different carbon sources in the nutrition medium on VOC emissions of micropropagated C. pulegium plants, using gas chromatography-mass spectrometry analysis of headspace VOCs. The volatile profiles were subjected to multivariate analysis with respect to the presence, concentration and type of carbon source in the nutrient medium. In addition, the effect of different carbohydrates on the density and size of the leaf glandular trichomes, the main structures involved in the emission of VOCs, was determined. A total of 19 VOCs, primarily belonging to mono- and sesquiterpenes previously described in plants, were tentatively identified. Six VOCs were produced at levels higher than 2% of the total VOC emission, dominated by pulegone, ss-pinene and menthone. Inclusion of the carbohydrates in the culture media affected the production of the main leaf trichome-associated volatile allelochemicals although the qualitative composition of the volatiles changed only slightly. Multivariate analysis showed that the concentration, rather than the carbohydrate type, influenced the VOC profile" |
| Keywords: | Clinopodium pulegium Gc-ms Lamiaceae VOC profile carbohydrates glandular trichomes headspace micropropagation terpenoids; |
| Notes: | "PubMed-not-MEDLINEStojicic, Dragana Tosic, Svetlana Stojanovic, Gordana Zlatkovic, Bojan Jovanovic, Snezana Budimir, Snezana Uzelac, Branka eng 451-03-9/2021-14/200007 and 451-03-9/2021-14/200124/Serbian Ministry of Education, Science and Technological Development/ Switzerland 2022/01/21 Plants (Basel). 2022 Jan 13; 11(2):198. doi: 10.3390/plants11020198" |
