| Title: | "Fuz1, a MYND domain protein, is required for cell morphogenesis in Ustilago maydis" |
| Author(s): | Chew E; Aweiss Y; Lu CY; Banuett F; |
| Address: | "Department of Chemistry and Biochemistry, California State University, 1250 Bellflower Boulevard, Long Beach, California 90840, USA" |
| DOI: | 10.3852/mycologia.100.1.31 |
| ISSN/ISBN: | 0027-5514 (Print) 1557-2536 (Electronic) 0027-5514 (Linking) |
| Abstract: | "Abstract: Ustilago maydis is a Basidiomycete fungus that exhibits a yeast-like nonpathogenic form and a dikaryotic filamentous pathogenic form. Generation of these two forms is controlled by two mating type loci, a and b. The fungus undergoes additional morphological transitions in the plant that result in formation of a third cell type, the teliospore. The fuz1 gene is necessary for this developmental program. Here we report cloning and sequencing of fuz1 and show that it contains an open reading frame with coding capacity for a protein of 1421 amino acids. The Fuz1 protein belongs to the family of MYND Zn finger domain proteins. We generate a null mutation in strains of opposite mating type and show that fuz1 is necessary for conjugation tube formation, a morphological transition that occurs in response to pheromones. We generate fuz1- diploid strains heterozygous at a and b and show that fuz1 is also necessary for postfusion events (maintenance of filamentous growth). We also demonstrate that fuz1 is necessary for cell morphogenesis of the yeast-like cell: normal cell length, location and number of septa, cell separation and constriction of the neck region. Fuz1 is also required for cell wall integrity and to prevent secretion of a dark pigment. We propose that the MYND domain may interact with different proteins to regulate cell morphogenesis" |
| Keywords: | "Amino Acid Sequence Cloning, Molecular Conjugation, Genetic Fungal Proteins/*chemistry/genetics/*metabolism Gene Expression Regulation, Fungal Molecular Sequence Data Mutation Pigments, Biological/metabolism Protein Structure, Tertiary Sequence Homology, ;" |
| Notes: | "MedlineChew, Emily Aweiss, Yara Lu, Ching-Yu Banuett, Flora eng S06 GM063119/GM/NIGMS NIH HHS/ S06 GM63119/GM/NIGMS NIH HHS/ HHMI/Howard Hughes Medical Institute/ S06 GM063119-06S10009/GM/NIGMS NIH HHS/ 2S06 GM063119/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't England 2008/05/21 Mycologia. 2008 Jan-Feb; 100(1):31-46. doi: 10.3852/mycologia.100.1.31" |
