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Mol Biol Cell

Title:		Oxidative stress activates FUS1 and RLM1 transcription in the yeast Saccharomyces cerevisiae in an oxidant-dependent Manner
Author(s):		Staleva L; Hall A; Orlow SJ;
Address:		"Department of Dermatology, New York University School of Medicine, New York, NY 10016, USA"
Journal Title:		Mol Biol Cell
Year:		2004
Volume:		20040922
Issue:		12
Page Number:		5574 - 5582
DOI:		10.1091/mbc.e04-02-0142
ISSN/ISBN:		1059-1524 (Print) 1059-1524 (Linking)
Abstract:		"Mating in haploid Saccharomyces cerevisiae occurs after activation of the pheromone response pathway. Biochemical components of this pathway are involved in other yeast signal transduction networks. To understand more about the coordination between signaling pathways, we used a 'chemical genetic' approach, searching for compounds that would activate the pheromone-responsive gene FUS1 and RLM1, a reporter for the cell integrity pathway. We found that catecholamines (l-3,4-hydroxyphenylalanine [l-dopa], dopamine, adrenaline, and noradrenaline) elevate FUS1 and RLM1 transcription. N-Acetyl-cysteine, a powerful antioxidant in yeast, completely reversed this effect, suggesting that FUS1 and RLM1 activation in response to catecholamines is a result of oxidative stress. The oxidant hydrogen peroxide also was found to activate transcription of an RLM1 reporter. Further genetic analysis combined with immunoblotting revealed that Kss1, one of the mating mitogen-activated protein kinases (MAPKs), and Mpk1, an MAPK of the cell integrity pathway, participated in l-dopa-induced stimulation of FUS1 and RLM1 transcription. We also report that Mpk1 and Hog1, the high osmolarity MAPK, were phosphorylated upon induction by hydrogen peroxide. Together, our results demonstrate that cells respond to oxidative stress via different signal transduction machinery dependent upon the nature of the oxidant"
Keywords:		"Cell Membrane Permeability Gene Expression Regulation, Fungal/*drug effects Hydrogen Peroxide/metabolism/pharmacology Levodopa/pharmacology MADS Domain Proteins MAP Kinase Signaling System/drug effects Membrane Proteins/genetics/metabolism Mitogen-Activat;"
Notes:		"MedlineStaleva, Liliana Hall, Andrea Orlow, Seth J eng R01 EY010223/EY/NEI NIH HHS/ EY10223/EY/NEI NIH HHS/ Research Support, U.S. Gov't, P.H.S. 2004/09/24 Mol Biol Cell. 2004 Dec; 15(12):5574-82. doi: 10.1091/mbc.e04-02-0142. Epub 2004 Sep 22"