| Title: | Wounding and Insect Feeding Trigger Two Independent MAPK Pathways with Distinct Regulation and Kinetics |
| Author(s): | Sozen C; Schenk ST; Boudsocq M; Chardin C; Almeida-Trapp M; Krapp A; Hirt H; Mithofer A; Colcombet J; |
| Address: | "Universite Paris-Saclay, CNRS, INRAE, Univ Evry, Institute of Plant Sciences Paris-Saclay (IPS2), 91405 Orsay, France. Institut Jean-Pierre Bourgin, INRAE, AgroParisTech, Universite Paris-Saclay, 78000 Versailles, France. Department of Bioorganic Chemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany. Center for Desert Agriculture, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia. Max F. Perutz Laboratories, University of Vienna, 1030 Vienna, Austria. Research Group Plant Defense Physiology, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany. Universite Paris-Saclay, CNRS, INRAE, Univ Evry, Institute of Plant Sciences Paris-Saclay (IPS2), 91405 Orsay, France jean.colcombet@inrae.fr" |
| ISSN/ISBN: | 1532-298X (Electronic) 1040-4651 (Print) 1040-4651 (Linking) |
| Abstract: | "Abiotic and biotic factors cause plant wounding and trigger complex short- and long-term responses at the local and systemic levels. These responses are under the control of complex signaling pathways, which are still poorly understood. Here, we show that the rapid activation of clade-A mitogen-activated protein kinases (MAPKs) MPK3 and MPK6 by wounding depends on the upstream MAPK kinases MKK4 and MKK5 but is independent of jasmonic acid (JA) signaling. In addition, this fast module does not control wound-triggered JA accumulation in Arabidopsis (Arabidopsis thaliana), unlike its orthologs in tobacco. We also demonstrate that a second MAPK module, composed of MKK3 and the clade-C MAPKs MPK1/2/7, is activated by wounding in a MKK4/5-independent manner. We provide evidence that the activation of this MKK3-MPK1/2/7 module occurs mainly through wound-induced JA production via the transcriptional regulation of upstream clade-III MAP3Ks, particularly MAP3K14. We show that mkk3 mutant plants are more susceptible to herbivory from larvae of the generalist lepidopteran herbivore Spodoptera littoralis, indicating that the MKK3-MPK1/2/7 module is involved in counteracting insect feeding" |
| Keywords: | "Animals Arabidopsis/genetics/*metabolism Arabidopsis Proteins/genetics/*metabolism Cyclopentanes/metabolism Gene Expression Regulation, Plant/genetics/physiology Kinetics Mitogen-Activated Protein Kinases/genetics/*metabolism Oxylipins/metabolism Signal T;" |
| Notes: | "MedlineSozen, Cecile Schenk, Sebastian T Boudsocq, Marie Chardin, Camille Almeida-Trapp, Marilia Krapp, Anne Hirt, Heribert Mithofer, Axel Colcombet, Jean eng Research Support, Non-U.S. Gov't England 2020/04/09 Plant Cell. 2020 Jun; 32(6):1988-2003. doi: 10.1105/tpc.19.00917. Epub 2020 Apr 7" |
