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Lipids

Title:		Expression of enzymes and transcription factors involved in n-3 long chain PUFA biosynthesis in limousin bull tissues
Author(s):		Cherfaoui M; Durand D; Bonnet M; Cassar-Malek I; Bauchart D; Thomas A; Gruffat D;
Address:		"INRA, UR 1213 Herbivore, Site de Theix, 63122, Saint-Genes-Champanelle, France"
Journal Title:		Lipids
Year:		2012
Volume:		20120108
Issue:		4
Page Number:		391 - 401
DOI:		10.1007/s11745-011-3644-z
ISSN/ISBN:		1558-9307 (Electronic) 0024-4201 (Linking)
Abstract:		"The current low consumption of n-3 long chain polyunsaturated fatty acids (n-3 LCPUFA) led scientists to wonder about the possible enrichment of human food, including meats such as beef, with n-3 LCPUFA. However, their biosynthesis from dietary n-3 PUFA seems limited in mammalian tissues implying that a better understanding of the molecular mechanisms responsible for this down regulation is needed. This study aimed at identifying and comparing the limiting steps of n-3 LCPUFA synthesis in liver, intermuscular adipose tissue (IM-AT) and semitendinosus muscle (ST) from six Limousin bulls. Tissue FA composition was analysed by GLC and mRNA abundance of enzymes and transcription factors involved in n-3 LCPUFA synthesis was assessed by RT-qPCR. In liver, mRNA encoding proteins involved in n-3 LCPUFA synthesis were present in agreement with the significant high content of n-3 LCPUFA (8.4 mol% of total FA, 257 mg/100 g of fresh tissue) in this organ. In IM-AT, these mRNA were all present, but at a tenfold lower intensity than in liver in agreement with the low contents of n-3 LCPUFA in this tissue. In ST muscle, these mRNA were all present except elongase 5 mRNA which was only present as trace, the corresponding protein being undetectable, probably inducing a break of n-3 LCPUFA synthesis from 18:4n-3. In conclusion, Limousin bull ST muscle seemed unable to synthesize n-3 LCPUFA. However, the presence of 20:5n-3 (EPA) and 22:5n-3 (DPAn-3) in muscle raised the question of the origin of these n-3 LCPUFA"
Keywords:		"Acyl-CoA Dehydrogenases/genetics/metabolism Acyltransferases/genetics/metabolism Adipose Tissue/*enzymology Animals Cattle Fatty Acid Desaturases/genetics/metabolism Fatty Acids, Omega-3/*biosynthesis Gene Expression Lipid Metabolism/*genetics Liver/*enzy;"
Notes:		"MedlineCherfaoui, Maya Durand, Denys Bonnet, Muriel Cassar-Malek, Isabelle Bauchart, Dominique Thomas, Agnes Gruffat, Dominique eng 2012/01/10 Lipids. 2012 Apr; 47(4):391-401. doi: 10.1007/s11745-011-3644-z. Epub 2012 Jan 8"