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Genetics

Title:		The ste3 pheromone receptor gene of Pneumocystis carinii is surrounded by a cluster of signal transduction genes
Author(s):		Smulian AG; Sesterhenn T; Tanaka R; Cushion MT;
Address:		"Infectious Disease Division, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0560, USA. smuliang@fuse.net"
Journal Title:		Genetics
Year:		2001
Volume:		157
Issue:		3
Page Number:		991 - 1002
DOI:		10.1093/genetics/157.3.991
ISSN/ISBN:		0016-6731 (Print) 0016-6731 (Linking)
Abstract:		"Although the clinical aspects of Pneumocystis carinii pneumonia are well characterized, the basic biology of the causative organism is poorly understood. Most proposed life cycles of P. carinii include both asexual and sexual replicative cycles. The two most prominent morphological forms are a trophic form, thought to undergo asexual replication by binary fission, and a cystic form or ascus containing intracystic bodies or ascospores, the products of sexual replication. To facilitate the Pneumocystis genome project, a P. carinii f. sp. carinii genomic cosmid library and an additional lambda cDNA library were generated. A partial expressed sequence tag database, created as part of the genome project, revealed the transcription of meiosis-specific genes and other genes related to sexual reproduction. The ortholog of Ste3, an a-factor pheromone receptor, was cloned and genes surrounding the ste3 locus were examined. Clustered around the ste3 gene are genes encoding elements functional in the pheromone response signal transduction cascade of model fungal organisms. These include the Ste20 protein kinase, the Ste12 homoeodomain transcriptional regulator, a potential pheromone mating factor, and other DNA-binding proteins. The genomic organization of the ste3 locus bears significant similarity to that of the mating locus recently described in Cryptococcus neoformans. The P. carinii genome contains much of the genetic machinery necessary for pheromone responsiveness, and these data support the existence of a sexual replication cycle"
Keywords:		"Amino Acid Sequence Blotting, Southern Cloning, Molecular Cosmids/genetics DNA, Complementary/metabolism Expressed Sequence Tags Gene Library *Genome, Fungal Models, Genetic Molecular Sequence Data *Multigene Family Pheromones/*genetics Pneumocystis/*gene;"
Notes:		"MedlineSmulian, A G Sesterhenn, T Tanaka, R Cushion, M T eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2001/03/10 Genetics. 2001 Mar; 157(3):991-1002. doi: 10.1093/genetics/157.3.991"