| Title: | Redundant actions of neuropeptides encoded by the dh-pban gene for larval color pattern formation in the oriental armyworm Mythimnaseparata |
| Author(s): | Shirai Y; Ono H; Daimon T; |
| Address: | "Department of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan. Department of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan. Electronic address: daimon.takaaki.7a@kyoto-u.ac.jp" |
| DOI: | 10.1016/j.ibmb.2023.103955 |
| ISSN/ISBN: | 1879-0240 (Electronic) 0965-1748 (Linking) |
| Abstract: | "The pyrokinin (PK)/pheromone biosynthesis-activating neuropeptide (PBAN) family, which is defined by a conserved C-terminal pentapeptide (FXPRLamide), is involved in many physiological processes in insects. In the oriental armyworm Mythimna separata, the larvae exhibit a variety of color patterns in response to changes in population density, which are caused by melanization and a reddish coloration hormone (MRCH), which is a member of the FXPRLamide neuropeptides. Interestingly, in some lepidopteran insects, MRCH is known as a PBAN, which activates the pheromone gland to produce sex pheromones. PBAN is encoded by a single gene, dh-pban, which encodes additional FXPRLamide neuropeptides, such as the diapause hormone (DH) and subesophageal ganglion neuropeptides (SGNPs). To determine the roles of the dh-pban gene, which produces multiple types of FXPRLamide neuropeptides after post-transcriptional cleavage of the precursor protein, we performed CRISPR/Cas9-mediated targeted mutagenesis in M. separata. We demonstrated that knockout armyworm larvae lost density-dependent cuticular melanization and retained yellow body color, even when reared under crowded conditions. Moreover, our rescue experiments using the synthetic peptides showed that not only PBAN but also beta- and gamma-SGNPs significantly induce the cuticular melanization in a dose dependent manner. Taken together, our results provide genetic evidence that neuropeptides encoded by the single dh-pban gene act redundantly to control density-dependent color pattern formation in M. separata" |
| Keywords: | Animals Larva/genetics/metabolism Spodoptera/metabolism *Moths/genetics/metabolism *Neuropeptides/genetics/metabolism *Sex Attractants/metabolism CRISPR/Cas9 Dh-pban FXPRLamide Mrch Mythimna separata Pban; |
| Notes: | "MedlineShirai, Yu Ono, Hajime Daimon, Takaaki eng Research Support, Non-U.S. Gov't England 2023/05/06 Insect Biochem Mol Biol. 2023 Jun; 157:103955. doi: 10.1016/j.ibmb.2023.103955. Epub 2023 May 3" |
