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J Plant Physiol

Title:		Quantitative analysis of herbivore-induced cytosolic calcium by using a Cameleon (YC 3.6) calcium sensor in Arabidopsis thaliana
Author(s):		Verrillo F; Occhipinti A; Kanchiswamy CN; Maffei ME;
Address:		"Plant Physiology Unit, Dept. Life Sciences and Systems Biology, University of Turin, Italy. Electronic address: francesca.verrillo@unito.it. Plant Physiology Unit, Dept. Life Sciences and Systems Biology, University of Turin, Italy. Electronic address: andrea.occhipinti@unito.it. Research and Innovation Center Biology and Genomic of Fruit Plants, Fondazione Edmund Mach FEM San Michele all'Adige (TN), Italy. Electronic address: chidubt@gmail.com. Plant Physiology Unit, Dept. Life Sciences and Systems Biology, University of Turin, Italy. Electronic address: massimo.maffei@unito.it"
Journal Title:		J Plant Physiol
Year:		2014
Volume:		20131120
Issue:		2
Page Number:		136 - 139
DOI:		10.1016/j.jplph.2013.09.020
ISSN/ISBN:		1618-1328 (Electronic) 0176-1617 (Linking)
Abstract:		"Ca(2+) is a key player in plant cell responses to biotic and abiotic stress. Owing to the central role of cytosolic Ca(2+) ([Ca(2+)]cyt) during early signaling and the need for precise determination of [Ca(2+)]cyt variations, we used a Cameleon YC 3.6 reporter protein expressed in Arabidopsis thaliana to quantify [Ca(2+)]cyt variations upon leaf mechanical damage (MD), herbivory by 3rd and 5th instar larvae of Spodoptera littoralis and S. littoralis oral secretions (OS) applied to MD. YC 3.6 allowed a clear distinction between MD and herbivory and discriminated between the two larvae instars. To our knowledge this is the first report of quantitative [Ca(2+)]cyt determination upon herbivory using a Cameleon calcium sensor"
Keywords:		Animals Arabidopsis/*metabolism Calcium/*metabolism Calcium Signaling/*physiology *Calmodulin Cytosol/metabolism *Herbivory *Luminescent Proteins *Recombinant Fusion Proteins Arabidopsis thaliana Calcium signaling Cameleon YC 3.6 Herbivory Spodoptera litt;
Notes:		"MedlineVerrillo, Francesca Occhipinti, Andrea Kanchiswamy, Chidananda Nagamangala Maffei, Massimo E eng Research Support, Non-U.S. Gov't Germany 2013/12/18 J Plant Physiol. 2014 Jan 15; 171(2):136-9. doi: 10.1016/j.jplph.2013.09.020. Epub 2013 Nov 20"