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J Microbiol Methods

Title:		Real-time detection of common microbial volatile organic compounds from medically important fungi by Selected Ion Flow Tube-Mass Spectrometry (SIFT-MS)
Author(s):		Scotter JM; Langford VS; Wilson PF; McEwan MJ; Chambers ST;
Address:		"Department of Pathology, Christchurch School of Medicine, University of Otago, Christchurch, New Zealand"
Journal Title:		J Microbiol Methods
Year:		2005
Volume:		63
Issue:		2
Page Number:		127 - 134
DOI:		10.1016/j.mimet.2005.02.022
ISSN/ISBN:		0167-7012 (Print) 0167-7012 (Linking)
Abstract:		"We describe a new method, Selected Ion Flow Tube-Mass Spectrometry (SIFT-MS) for the rapid and sensitive real-time detection and quantification of volatile organic compounds from medically important fungi, grown on a range of laboratory media. SIFT-MS utilises the chemical ionisation reactions of mass-selected ions to characterise volatile organic compounds (VOCs) that are produced as metabolites from fungi. This technique has the distinct advantage over others in that it readily detects low molecular weight, reactive volatiles, and allows for real-time, quantitative monitoring. The fungi examined in this study were Aspergillus flavus, Aspergillus fumigatus, Candida albicans, Mucor racemosus, Fusarium solani, and Cryptococcus neoformans grown on or in malt extract agar, Columbia agar, Sabouraud's dextrose agar, blood agar, and brain-heart infusion broth. Common metabolites (ethanol, methanol, acetone, acetaldehyde, methanethiol, and crotonaldehyde) were detected and quantified. We found the fingerprint of volatiles, in terms of presence and quantity of volatiles to be strongly dependent on the culture medium, both in terms of variety and quantity of volatiles produced, but may form the basis for species specific identification of medically important fungi"
Keywords:		Culture Media Humans Mass Spectrometry/methods Mitosporic Fungi/*classification/*growth & development/isolation & purification/metabolism Mucor/*classification/*growth & development/isolation & purification/metabolism Mycological Typing Techniques Mycoses;
Notes:		"MedlineScotter, Jennifer M Langford, Vaughan S Wilson, Paul F McEwan, Murray J Chambers, Stephen T eng Evaluation Study Research Support, Non-U.S. Gov't Netherlands 2005/05/17 J Microbiol Methods. 2005 Nov; 63(2):127-34. doi: 10.1016/j.mimet.2005.02.022"