| Title: | Thiamin-dependent enzymes as catalysts in chemoenzymatic syntheses |
| Address: | "Institut fur Biotechnologie 1, Forschungszentrum Julich GmbH, P.O. Box 1913, D-52425 Julich, Germany" |
| DOI: | 10.1016/s0167-4838(98)00071-5 |
| ISSN/ISBN: | 0006-3002 (Print) 0006-3002 (Linking) |
| Abstract: | "Enzymes are increasingly being used to perform regio- and enantioselective reactions in chemoenzymatic syntheses. To utilize enzymes for unphysiological reactions and to yield novel products, a broad substrate spectrum is desirable. Thiamin diphosphate (ThDP)-dependent enzymes vary in their substrate tolerance from rather strict substrate specificity (phosphoketolases, glyoxylate carboligase) to more permissive enzymes (transketolase, dihydroxyacetone synthase, pyruvate decarboxylase) and therefore differ in their potential to be used as biocatalysts. We give an overview of the known substrate spectra of ThDP-dependent enzymes and present examples of multi-enzyme or chemoenzymatic approaches which involve ThDP-dependent enzymes as biocatalysts to obtain pharmaceutical compounds as ephedrine and glycosidase inhibitors, sex pheromones as exo-brevicomin, 13C-labeled metabolites, and other intermediates as 1-deoxyxylulose 5-phosphate, a precursor of vitamins and isoprenoids" |
| Keywords: | Aldehyde-Ketone Transferases/chemistry/metabolism Carboxy-Lyases/chemistry/metabolism Catalysis Pharmaceutical Preparations/*chemical synthesis Pyruvate Decarboxylase/chemistry/metabolism Pyruvate Dehydrogenase Complex/chemistry/metabolism Stereoisomerism; |
| Notes: | "MedlineSchorken, U Sprenger, G A eng Research Support, Non-U.S. Gov't Review Netherlands 1998/07/10 Biochim Biophys Acta. 1998 Jun 29; 1385(2):229-43. doi: 10.1016/s0167-4838(98)00071-5" |
