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J Sci Food Agric

Title:		Repeated measurements of in vitro fermentation of fibre-rich substrates using large intestinal microbiota of sows
Author(s):		Sappok MA; Pellikaan WF; Verstegen MW; Bosch G; Sundrum A; Hendriks WH;
Address:		"Animal Nutrition Group, Department of Animal Sciences, University of Wageningen, 6700 AH Wageningen, The Netherlands. maria.sappok@yahoo.de"
Journal Title:		J Sci Food Agric
Year:		2013
Volume:		20120830
Issue:		5
Page Number:		987 - 994
DOI:		10.1002/jsfa.5834
ISSN/ISBN:		1097-0010 (Electronic) 0022-5142 (Linking)
Abstract:		"BACKGROUND: Fibrous ingredients for pig diets can be characterized by in vitro fermentation. In vitro fermentation methods often use a one-time measurement of gas production during the incubation of test substrates with one faecal inoculum. The representativeness of this approach can be questioned as measuring time and number of animals from which inoculum originates may influence fermentation results. An in vitro fermentation trial was conducted incubating three fibrous substrates with three inocula in five replicates (different fermentation runs) to test the influence of run and origin of inocula. RESULTS: Total gas production and maximal rate of gas production differed (P < 0.05) between fermentation runs, but less than substrates (P < 0.01). The ranking order between substrates remained similar for each run. Fermentation of cellulose led to higher coefficients of variation between inocula compared to the fast fermentable substrates oligofructose and soy pectin. Differences ranged from 2% for total gas production up to 25% for maximal rate of gas production. CONCLUSION: One fermentation run can provide representative results for substrate ranking. Using multiple inocula mixed from four faecal samples each leads to high coefficients of variation for slow fermentable substrates like cellulose. Future studies should examine the optimal number of animals for inocula preparation to decrease variation"
Keywords:		"Ammonia/analysis/metabolism Animal Feed/*analysis Animals Cellulose/administration & dosage/analysis/metabolism Crosses, Genetic Diet/*veterinary Dietary Fiber/administration & dosage/analysis/*metabolism Feces/microbiology Female Fermentation Gram-Negati;"
Notes:		"MedlineSappok, Maria A Pellikaan, Wilbert F Verstegen, Martin W A Bosch, Guido Sundrum, Albert Hendriks, Wouter H eng Comparative Study England 2012/09/01 J Sci Food Agric. 2013 Mar 30; 93(5):987-94. doi: 10.1002/jsfa.5834. Epub 2012 Aug 30"