| Title: | Linalool- and alpha-terpineol-induced programmed cell death in Chlamydomonas reinhardtii |
| Author(s): | Chen Y; Weng Y; Zhou M; Meng Y; Liu J; Yang L; Zuo Z; |
| Address: | "School of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, China. Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin 300387, China. School of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, China. Electronic address: zuozhaojiang@126.com" |
| DOI: | 10.1016/j.ecoenv.2018.10.062 |
| ISSN/ISBN: | 1090-2414 (Electronic) 0147-6513 (Linking) |
| Abstract: | "Plant allelochemicals effectively inhibit and/ or control algal growth, and have potential to use as algaecide. To uncover the lethal mechanism of 2 anti-algal compounds linalool and alpha-terpineol identified from Cinnamomum camphora extracts, and promote their development as algaecide, the H(2)O(2) production, photosynthetic abilities, caspase-like activities, nuclear changes and DNA degradation were investigated in Chlamydomonas reinhardtii treated with the 2 compounds. H(2)O(2) content burst in linalool treatment at 0.5?ª+h and in alpha-terpineol treatment at 1?ª+h, with increases of 2.7 folds and 1.3 folds, respectively, compared to that at 0?ª+h. The photosynthetic pigments gradually degraded, and Fv/Fm gradually declined to zero, indicating that the cell death was not a necrosis due to the gradual disappearance of physiological process. In C. reinhardtii cells, the caspase-9-like and caspase-3-like were activated in the treatments with the 2 compounds for 1?ª+h. With prolonging the treatment time, the fluorescent intensity of the cell nucleuses stained by DAPI gradually enhanced and then faded, and the genomic DNA isolated from the cells gradually degraded. These hallmarks indicated that the death of C. reinhardtii cells in linalool and alpha-terpineol treatments was a programmed cell death (PCD) triggered by the increased reactive oxygen species (ROS). Compared to alpha-terpineol treatment, linalool treatment showed stronger promoting effects on PCD at the same time point, which may be caused by the higher ROS content inducing higher caspase-9-like and caspase-3-like activities in a short time" |
| Keywords: | Acyclic Monoterpenes Apoptosis/*drug effects Caspase 3/metabolism Caspase 9/metabolism Chlamydomonas reinhardtii/*cytology Cyclohexane Monoterpenes Cyclohexenes/*toxicity Herbicides/toxicity Hydrogen Peroxide/metabolism Monoterpenes/*toxicity Pheromones/t; |
| Notes: | "MedlineChen, Yueting Weng, Yuanyuan Zhou, Min Meng, Yiyu Liu, Jialu Yang, Lin Zuo, Zhaojiang eng Netherlands 2018/10/28 Ecotoxicol Environ Saf. 2019 Jan 15; 167:435-440. doi: 10.1016/j.ecoenv.2018.10.062. Epub 2018 Oct 24" |
