| Title: | Specificity of MAP kinase signaling in yeast differentiation involves transient versus sustained MAPK activation |
| Author(s): | Sabbagh W; Flatauer LJ; Bardwell AJ; Bardwell L; |
| Address: | "Department of Developmental and Cell Biology, University of California, Irvine, Irvine, CA 92697, USA" |
| DOI: | 10.1016/s1097-2765(01)00322-7 |
| ISSN/ISBN: | 1097-2765 (Print) 1097-4164 (Electronic) 1097-2765 (Linking) |
| Abstract: | "Signals transmitted by common components often elicit distinct (yet appropriate) outcomes. In yeast, two developmental options-mating and invasive growth-are both regulated by the same MAP kinase cascade. Specificity has been thought to result from specialized roles for the two MAP kinases, Kss1 and Fus3, and because Fus3 prevents Kss1 from gaining access to the mating pathway. Kss1 has been thought to participate in mating only when Fus3 is absent. Instead, we show that Kss1 is rapidly phosphorylated and potently activated by mating pheromone in wild-type cells, and that this is required for normal pheromone-induced gene expression. Signal identity is apparently maintained because active Fus3 limits the extent of Kss1 activation, thereby preventing inappropriate signal crossover" |
| Keywords: | "Cell Differentiation/genetics/physiology Fungal Proteins/genetics/*metabolism Gene Expression Regulation, Fungal Genes, Reporter/genetics MAP Kinase Kinase Kinases/metabolism MAP Kinase Signaling System/*physiology Mitogen-Activated Protein Kinase Kinases;" |
| Notes: | "MedlineSabbagh, W Jr Flatauer, L J Bardwell, A J Bardwell, L eng GM07311/GM/NIGMS NIH HHS/ GM60366/GM/NIGMS NIH HHS/ T32 GM007311/GM/NIGMS NIH HHS/ R01 GM060366/GM/NIGMS NIH HHS/ R01 GM060366-01/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 2001/10/05 Mol Cell. 2001 Sep; 8(3):683-91. doi: 10.1016/s1097-2765(01)00322-7" |
