| Title: | Wound-induced oxidative responses in mountain birch leaves |
| Address: | "Section of Ecology, Department of Biology, University of Turku, FIN-20014 Turku, Finland. teiruu@utu.fi" |
| ISSN/ISBN: | 0305-7364 (Print) 1095-8290 (Electronic) 0305-7364 (Linking) |
| Abstract: | "AIMS: The aim of the study was to examine oxidative responses in subarctic mountain birch, Betula pubescens subsp. czerepanovii, induced by herbivory and manual wounding. METHODS: Herbivory-induced changes in polyphenoloxidase, peroxidase and catalase activities in birch leaves were determined. A cytochemical dye, 3,3-diaminobenzidine, was used for the in situ and in vivo detection of H2O2 accumulation as a response to herbivory and wounding. To localize peroxidase activity in leaves, 10 mm H2O2 was applied to the dye reagent. KEY RESULTS: Feeding by autumnal moth, Epirrita autumnata, larvae caused an induction in polyphenoloxidase and peroxidase activities within 24 h, and a concomitant decrease in the activity of antioxidative catalases in wounded leaves. Wounding also induced H2O2 accumulation, which may have both direct and indirect defensive properties against herbivores. Wound sites and guard cells showed a high level of peroxidase activity, which may efficiently restrict invasion by micro-organisms. CONCLUSION: Birch oxidases together with their substrates may form an important front line in defence against herbivores and pathogens" |
| Keywords: | Animals Betula/anatomy & histology/*enzymology/metabolism Catalase/metabolism Catechol Oxidase/*metabolism Finland Hydrogen Peroxide/metabolism Moths/pathogenicity Oxidation-Reduction Peroxidases/*metabolism Plant Leaves/anatomy & histology/*enzymology/me; |
| Notes: | "MedlineRuuhola, Teija Yang, Shiyong eng Research Support, Non-U.S. Gov't England 2005/10/29 Ann Bot. 2006 Jan; 97(1):29-37. doi: 10.1093/aob/mcj005. Epub 2005 Oct 27" |
