| Title: | Volatile Organic Compound Detection by Graphene Field-Effect Transistors Functionalized with Fly Olfactory Receptor Mimetic Peptides |
| Author(s): | Rungreungthanapol T; Homma C; Akagi KI; Tanaka M; Kikuchi J; Tomizawa H; Sugizaki Y; Isobayashi A; Hayamizu Y; Okochi M; |
| Address: | "Department of Chemical Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology, 2-12-1 Ookayama, Meguro-ku, Tokyo 152-8552, Japan. Department of Materials Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology, 2-12-1 Ookayama, Meguro-ku, Tokyo 152-8552, Japan. Environmental Metabolic Analysis Research Team, RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro, Tsurumi, Yokohama, Kanagawa 230-0045, Japan. Department of Chemical Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama-shi, Kanagawa 226-8503, Japan. Corporate Research & Development Center, Toshiba Corporation, 1, Komukai-Toshiba-Cho, Saiwai-ku, Kawasaki 212-8583, Japan" |
| DOI: | 10.1021/acs.analchem.3c00052 |
| ISSN/ISBN: | 1520-6882 (Electronic) 0003-2700 (Linking) |
| Abstract: | "An olfactory receptor mimetic peptide-modified graphene field-effect transistor (gFET) is a promising solution to overcome the principal challenge of low specificity graphene-based sensors for volatile organic compound (VOC) sensing. Herein, peptides mimicking a fruit fly olfactory receptor, OR19a, were designed by a high-throughput analysis method that combines a peptide array and gas chromatography for the sensitive and selective gFET detection of the signature citrus VOC, limonene. The peptide probe was bifunctionalized via linkage of a graphene-binding peptide to facilitate one-step self-assembly on the sensor surface. The limonene-specific peptide probe successfully achieved highly sensitive and selective detection of limonene by gFET, with a detection range of 8-1000 pM, while achieving facile sensor functionalization. Taken together, our target-specific peptide selection and functionalization strategy of a gFET sensor demonstrates advancement of a precise VOC detection system" |
| Keywords: | "*Biosensing Techniques/methods *Graphite/chemistry Limonene Peptides *Receptors, Odorant Transistors, Electronic *Volatile Organic Compounds/analysis Drosophila Animals;" |
| Notes: | "MedlineRungreungthanapol, Tharatorn Homma, Chishu Akagi, Ken-Ichi Tanaka, Masayoshi Kikuchi, Jun Tomizawa, Hideyuki Sugizaki, Yoshiaki Isobayashi, Atsunobu Hayamizu, Yuhei Okochi, Mina eng Research Support, Non-U.S. Gov't 2023/02/22 Anal Chem. 2023 Mar 7; 95(9):4556-4563. doi: 10.1021/acs.analchem.3c00052. Epub 2023 Feb 20" |
