| Title: | Yeast alpha 2 repressor positions nucleosomes in TRP1/ARS1 chromatin |
| Author(s): | Roth SY; Dean A; Simpson RT; |
| Address: | "Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892" |
| DOI: | 10.1128/mcb.10.5.2247-2260.1990 |
| ISSN/ISBN: | 0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking) |
| Abstract: | "The yeast alpha 2 repressor suppresses expression of a-mating-type-specific genes in haploid alpha and diploid a/alpha cell types. We inserted the alpha 2-binding site into the multicopy TRP1/ARS1 yeast plasmid and examined the effects of alpha 2 on the chromatin structure of the derivative plasmids in alpha cells, and a/alpha cells. Whereas no effect on nucleosome position was observed in a cells, nucleosomes were precisely and stably positioned over sequences flanking the alpha 2 operator in alpha and a/alpha cells. In addition, when the alpha 2 operator was located upstream of the TRP1 gene, an extended array of positioned nucleosomes was formed in alpha cells and a/alpha cells, with formation of a nucleosome not present in a cells, and TRP1 mRNA production was substantially reduced. These data indicate that alpha 2 causes a positioning of nucleosomes over sequences proximal to its operator in TRP1/ARS1 chromatin and suggest that changes in chromatin structure may be related to alpha 2 repression of cell-type-specific genes" |
| Keywords: | "Gene Expression Regulation, Fungal Mating Factor Micrococcal Nuclease/pharmacology Nucleosomes/*ultrastructure *Operator Regions, Genetic Peptides/physiology Plasmids Regulatory Sequences, Nucleic Acid Repressor Proteins/*physiology Saccharomyces cerevisi;" |
| Notes: | "MedlineRoth, S Y Dean, A Simpson, R T eng 1990/05/01 Mol Cell Biol. 1990 May; 10(5):2247-60. doi: 10.1128/mcb.10.5.2247-2260.1990" |
