| Title: | Signaling and circuitry of multiple MAPK pathways revealed by a matrix of global gene expression profiles |
| Author(s): | Roberts CJ; Nelson B; Marton MJ; Stoughton R; Meyer MR; Bennett HA; He YD; Dai H; Walker WL; Hughes TR; Tyers M; Boone C; Friend SH; |
| Address: | "Rosetta Inpharmatics, 12040 115th Avenue Northeast, Kirkland, WA 98034, USA" |
| DOI: | 10.1126/science.287.5454.873 |
| ISSN/ISBN: | 0036-8075 (Print) 0036-8075 (Linking) |
| Abstract: | "Genome-wide transcript profiling was used to monitor signal transduction during yeast pheromone response. Genetic manipulations allowed analysis of changes in gene expression underlying pheromone signaling, cell cycle control, and polarized morphogenesis. A two-dimensional hierarchical clustered matrix, covering 383 of the most highly regulated genes, was constructed from 46 diverse experimental conditions. Diagnostic subsets of coexpressed genes reflected signaling activity, cross talk, and overlap of multiple mitogen-activated protein kinase (MAPK) pathways. Analysis of the profiles specified by two different MAPKs-Fus3p and Kss1p-revealed functional overlap of the filamentous growth and mating responses. Global transcript analysis reflects biological responses associated with the activation and perturbation of signal transduction pathways" |
| Keywords: | "*Cell Cycle Proteins Cyclin-Dependent Kinase Inhibitor Proteins Fungal Proteins/genetics/metabolism/physiology G1 Phase *Gene Expression Profiling *Gene Expression Regulation, Fungal Genome, Fungal Lipoproteins/pharmacology/physiology *MAP Kinase Signalin;" |
| Notes: | "MedlineRoberts, C J Nelson, B Marton, M J Stoughton, R Meyer, M R Bennett, H A He, Y D Dai, H Walker, W L Hughes, T R Tyers, M Boone, C Friend, S H eng Research Support, Non-U.S. Gov't 2000/02/05 Science. 2000 Feb 4; 287(5454):873-80. doi: 10.1126/science.287.5454.873" |
