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Environ Sci Pollut Res Int

Title:		Predicting the insecticide-driven mutations in a crop pest insect: Evidence for multiple polymorphisms of acetylcholinesterase gene with potential relevance for resistance to chemicals
Author(s):		Renault D; Elfiky A; Mohamed A;
Address:		"University of Rennes, CNRS, ECOBIO (Ecosystemes, Biodiversite, Evolution), UMR, 6553, Rennes, France. david.renault@univ-rennes1.fr. Institut Universitaire de France, 1 rue Descartes, 75231, Paris Cedex 05, France. david.renault@univ-rennes1.fr. Biophysics Department, Faculty of Science, Cairo University, Giza, 12613, Egypt. Department of Entomology, Faculty of Science, Cairo University, Giza, 12613, Egypt"
Journal Title:		Environ Sci Pollut Res Int
Year:		2023
Volume:		20221011
Issue:		7
Page Number:		18937 - 18955
DOI:		10.1007/s11356-022-23309-w
ISSN/ISBN:		1614-7499 (Electronic) 0944-1344 (Linking)
Abstract:		"The silverleaf whitefly Bemisia tabaci (Gennadius, 1889) (Homoptera: Aleyrodidae) is a serious invasive herbivorous insect pest worldwide. The excessive use of pesticides has progressively selected B. tabaci specimens, reducing the effectiveness of the treatments, and ultimately ending in the selection of pesticide-resistant strains. The management of this crop pest has thus become challenging owing to the level of resistance to all major classes of recommended insecticides. Here, we used in silico techniques for detecting sequence polymorphisms in ace1 gene from naturally occurring B. tabaci variants, and monitor the presence and frequency of the detected putative mutations from 30 populations of the silverleaf whitefly from Egypt and Pakistan. We found several point mutations in ace1-type acetylcholinesterase (ace1) in the studied B. tabaci variants naturally occurring in the field. By comparing ace1 sequence data from an organophosphate-susceptible and an organophosphate-resistant strains of B. tabaci to ace1 sequence data retrieved from GenBank for that species and to nucleotide polymorphisms from other arthropods, we identified novel mutations that could potentially influence insecticide resistance. Homology modeling and molecular docking analyses were performed to determine if the mutation-induced changes in form 1 acetylcholinesterase (AChE1) structure could confer resistance to carbamate and organophosphate insecticides. Mutations had small effects on binding energy (DeltaG(b)) interactions between mutant AChE1 and insecticides; they altered the conformation of the peripheral anionic site of AChE1, and modified the enzyme surface, and these changes have potential effects on the target-site sensitivity. Altogether, the results from this study provide information on genic variants of B. tabaci ace1 for future monitoring insecticide resistance development and report a potential case of environmentally driven gene variations"
Keywords:		Animals *Insecticides/pharmacology/metabolism Acetylcholinesterase/genetics/metabolism Molecular Docking Simulation Mutation *Pesticides/metabolism *Hemiptera/genetics Insecticide Resistance/genetics Organophosphates/pharmacology Acetylcholinesterase Envi;
Notes:		"MedlineRenault, David Elfiky, Abdo Mohamed, Amr eng Germany 2022/10/12 Environ Sci Pollut Res Int. 2023 Feb; 30(7):18937-18955. doi: 10.1007/s11356-022-23309-w. Epub 2022 Oct 11"