| Title: | Characterization of Non-coding Regions in B Mating Loci of Agrocybe salicacola Groups: Target Sites for B Mating Type Identification |
| Author(s): | Chen W; Chai H; Yang W; Zhang X; Chen Y; Zhao Y; |
| Address: | "Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650223, Yunnan, China. Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming, 650223, Yunnan, China. Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, Kunming, 650223, Yunnan, China. College of Life Science, Southwest Forestry University, Kunming, 650224, Yunnan, China. Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650223, Yunnan, China. yaasmushroom@aliyun.com. Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming, 650223, Yunnan, China. yaasmushroom@aliyun.com. Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, Kunming, 650223, Yunnan, China. yaasmushroom@aliyun.com" |
| DOI: | 10.1007/s00284-017-1247-5 |
| ISSN/ISBN: | 1432-0991 (Electronic) 0343-8651 (Linking) |
| Abstract: | "Agrocybe salicacola is a delicious and cultivable mushroom. It is important to understand this species' inherent characteristics, especially to elucidate the constitution and segregation of mating genes. In this study, two compatible B mating loci in strain YAASM0711 of A. salicacola were cloned from the monokaryons, and sequence and phylogeny analyses showed two conserved genes encoding pheromone receptors maybe lost mating activity, which determined by comparing with those of other mushrooms. In the conserved regions of mating loci, partial insertion/deletion fragments made non-coding regions posses polymorphisms, and monokaryotic strains of different mating types were distinguished from each other according to the amplification profile of variable regions, which suggested mating loci were integrally assigned to offspring strains during mitosis in A. salicacola. As our known, it is the first to develop molecular markers for B mating-type identification using variable non-coding fragments of mating loci in basidiomycetes" |
| Keywords: | "Agrocybe/classification/*genetics Base Sequence Cloning, Molecular Conserved Sequence/genetics Fungal Proteins/*genetics Genes, Mating Type, Fungal/*genetics Phylogeny Polymorphism, Genetic/genetics RNA, Untranslated/*genetics Receptors, Pheromone/*geneti;" |
| Notes: | "MedlineChen, Weimin Chai, Hongmei Yang, Weixian Zhang, Xiaolei Chen, Yuhui Zhao, YongChang eng 2017/04/11 Curr Microbiol. 2017 Jun; 74(6):772-778. doi: 10.1007/s00284-017-1247-5. Epub 2017 Apr 10" |
