| Title: | "A pheromonotropic peptide of Helicoverpa zea, with melanizing activity, interaction with PBAN, and distribution of immunoreactivity" |
| Author(s): | Raina AK; Kingan TG; Kochansky JP; |
| Address: | "Formosan Subterranean Termite Research unit, USDA, ARS, New Orleans, Louisiana 70179, USA. araina@srr.ars.usda.gov" |
| Journal Title: | Arch Insect Biochem Physiol |
| ISSN/ISBN: | 0739-4462 (Print) 0739-4462 (Linking) |
| Abstract: | "The sequence of an 18-amino acid residue peptide was deduced from the gene encoding PBAN and other peptides with common C-termini in Helicoverpa zea. The peptide caused melanization in larvae and pheromone production in females of H. zea, and was designated pheromonotropic melanizing peptide (Hez-PMP). The peptide has a 83% sequence homology with a pheromonotropic peptide isolated from Pseudaletia separata. PMP caused melanization and mortality when injected into larvae just before molting. Whereas intense melanization was caused with a dose of 1,000 pmol, peak mortality occurred at 100 pmol, with 50% of larvae dying within 48 h after injection. Pheromonotropic activity of PMP was dose dependent. Co-injection of Hez-PMP and Hez-PBAN into a female resulted in suppression of the pheromonotropic effect of PBAN. Whole-mount immunocytochemical studies revealed PMP-like immunoreactivity in frontal ganglion, subesophageal, thoracic, and abdominal ganglia as well as the esophageal nerve" |
| Keywords: | "Amino Acid Sequence Animals Dose-Response Relationship, Drug Female Ganglia/cytology/metabolism/ultrastructure Immunohistochemistry/methods Larva/drug effects Lepidoptera/chemistry/genetics/*metabolism Melanins/biosynthesis Molecular Sequence Data Neurope;" |
| Notes: | "MedlineRaina, Ashok K Kingan, Timothy G Kochansky, Jan P eng 2003/07/30 Arch Insect Biochem Physiol. 2003 Aug; 53(4):147-57. doi: 10.1002/arch.10094" |
