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Arch Insect Biochem Physiol

Title:		Spatial and temporal distribution of pheromone biosynthesis-activating neuropeptide in Helicoverpa (Heliothis) armigera using RIA and in vitro bioassay
Author(s):		Rafaeli A; Hirsch J; Soroker V; Kamensky B; Raina A;
Address:		"Hebrew University, Department of Entomology, Rehovot, Israel"
Journal Title:		Arch Insect Biochem Physiol
Year:		1991
Volume:		18
Issue:		2
Page Number:		119 - 129
DOI:		10.1002/arch.940180206
ISSN/ISBN:		0739-4462 (Print) 0739-4462 (Linking)
Abstract:		"A [3H]-PBAN (pheromone biosynthesis-activating neuropeptide) analog was synthesized, and binding of the radioligand to a specific PBAN-antiserum was achieved. The inhibition of binding of the radioligand by unlabeled PBAN, several PBAN analogs, and other competitors was studied and a specific radio-immunoassay was developed. Using this radioimmunoassay we found PBAN-like immunoreactivity in methanol extracts of hemolymph and neural tissues from females. Higher levels of PBAN-like immunoreactivity in extracts of brain-suboesophageal ganglion complexes, corpora cardiaca, thoracic ganglia, and abdominal ganglia were observed during the 4-5th h scotophase when compared to the PBAN-like immunoactivity levels during the 6-11th h photophase. On the other hand, the concentrations of PBAN-like immunoreactivity, in the terminal abdominal ganglion were higher during the photophase relative to minimal levels observed during the scotophase, indicating an accumulation before the onset of pheromone production. These differences in concentrations of PBAN were also reflected in the stimulation of in vitro pheromone glands, whereby significant stimulations were obtained by scotophase and photophase brain extracts, scotophase thoracic ganglia extracts, and photophase terminal abdominal ganglia extracts. No detectable levels of PBAN were found in hemolymph extracts during the sampling periods"
Keywords:		Amino Acid Sequence Animals Female In Vitro Techniques Molecular Sequence Data Moths/*metabolism Neuropeptides/*biosynthesis Radioimmunoassay Sex Attractants/biosynthesis;
Notes:		"MedlineRafaeli, A Hirsch, J Soroker, V Kamensky, B Raina, A eng Research Support, Non-U.S. Gov't 1991/01/01 Arch Insect Biochem Physiol. 1991; 18(2):119-29. doi: 10.1002/arch.940180206"