| Title: | "STE50, a novel gene required for activation of conjugation at an early step in mating in Saccharomyces cerevisiae" |
| Author(s): | Rad MR; Xu G; Hollenberg CP; |
| Address: | "Institut fur Mikrobiologie, Heinrich-Heine-Universitat, Dusseldorf 1, FRG" |
| ISSN/ISBN: | 0026-8925 (Print) 0026-8925 (Linking) |
| Abstract: | "A new gene, STE50, which plays an essential role in cell differentiation in Saccharomyces cerevisiae was detected and analysed. STE50 expression is not cell type-specific and its expression in MATa and MAT alpha cells is unaffected by pheromones. When present on a high copy number plasmid, STE50 causes supersensitivity to alpha-pheromone, and increases the level of alpha-pheromone-induced transcription of FUS1 in haploid a cells. Mutants bearing either of the two gene disruptions, ste50-1 or ste50-2, are sterile and have a modulated sensitivity to alpha-pheromone. The overexpression of STE4 (G beta) in wild-type cells elicits a constitutive growth arrest signal, however this phenotype is suppressed by a C-terminal truncation mutation in STE50 (ste50-2). In contrast, the constitutive activation of the pheromone response pathway caused by disruption of GPA1 (G alpha) is not suppressed in ste50-2 mutants. The ste50-2 mutation partially suppresses the desensitisation defect of the sst2-1 mutation, and the resulting ste50-2 sst2-1 mutants restore fertility. Our results indicate that the ste50-2 mutant may have a defect in adaptation (hyperadaptation), and suggest a possible interaction of STE50-2 with the G alpha subunit of the G protein" |
| Keywords: | "Amino Acid Sequence Base Sequence *Conjugation, Genetic DNA, Recombinant Fungal Proteins/*genetics *GTP-Binding Protein beta Subunits GTP-Binding Proteins/genetics/metabolism Gene Expression *Genes, Fungal *Heterotrimeric GTP-Binding Proteins Molecular Se;" |
| Notes: | "MedlineRad, M R Xu, G Hollenberg, C P eng Research Support, Non-U.S. Gov't Germany 1992/12/01 Mol Gen Genet. 1992 Dec; 236(1):145-54. doi: 10.1007/BF00279653" |
