| Title: | Regulation of the pAD1-encoded sex pheromone response in Enterococcus faecalis: nucleotide sequence analysis of traA |
| Address: | "Department of Microbiology and Immunology, School of Medicine, University of Michigan, Ann Arbor 48109-0402" |
| DOI: | 10.1128/jb.174.6.1821-1827.1992 |
| ISSN/ISBN: | 0021-9193 (Print) 1098-5530 (Electronic) 0021-9193 (Linking) |
| Abstract: | "The Enterococcus faecalis plasmid pAD1 conjugatively transfers in response to a sex pheromone, cAD1, excreted by potential recipient cells. A key determinant responsible for regulation of pAD1 transfer is traA, which encodes a negative regulator also believed to function in signal sensing. In this study, we analyzed the nucleotide sequence and transcription of traA. A protein of 319 amino acids with a molecular weight of 37,856 was inferred and found to exhibit limited homology with several DNA-binding proteins. Analysis of Tn917-lac insertions resulting in transcriptional lacZ fusions within the 3' end of the traA transcript showed that it overlaps slightly with a convergently-transcribed C-region transcript. Insertional mutations affecting TraA repressor function and signal sensing functions were localized" |
| Keywords: | "Amino Acid Sequence Bacterial Outer Membrane Proteins/*genetics Base Sequence DNA Transposable Elements DNA, Bacterial/genetics Enterococcus faecalis/*genetics Fimbriae Proteins *Gene Expression Regulation, Bacterial Genes, Bacterial Molecular Sequence Da;" |
| Notes: | "MedlinePontius, L T Clewell, D B eng AI10318/AI/NIAID NIH HHS/ GM33956/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. 1992/03/01 J Bacteriol. 1992 Mar; 174(6):1821-7. doi: 10.1128/jb.174.6.1821-1827.1992" |
