| Title: | The effect of protocatechuic acid on the phycosphere in harmful algal bloom species Scrippsiella trochoidea |
| Author(s): | Chen Q; Zhu B; Sun D; Liu W; Sun X; Duan S; |
| Address: | "Department of Ecology, Jinan University, Guangzhou 510632, China. School of Life Science and Engineering, State Defense Key Laboratory of the Nuclear Waste and Environmental Security, Southwest University of Science and Technology, Mianyang 621010, China. South China Institute of Environmental Science, Ministry of Ecology and Environment of the People's Republic of China, Guangzhou 510530, China. Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai Key Laboratory of Marine Bioresources and Environment, Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510275, China. Electronic address: sunx27@mail.sysu.edu.cn. Department of Ecology, Jinan University, Guangzhou 510632, China. Electronic address: tssduan@jnu.edu.cn" |
| DOI: | 10.1016/j.aquatox.2020.105591 |
| ISSN/ISBN: | 1879-1514 (Electronic) 0166-445X (Linking) |
| Abstract: | "The effects of allelopathy and the potential harm of several isolated allelochemicals have been studied in detail. Microorganisms in the phycosphere play an important role in algal growth, decay and nutrient cycling. However, it is unknown and often neglected whether allelochemicals affect the phycosphere. The present study selected a phenolic acid protocatechuic acid (PA) - previously shown to be an allelochemical. We studied PA at a half maximal effective concentration of 0.20 mM (30 mg L(-1)) against Scrippsiella trochoidea to assess the effect of PA on its phycosphere in an acute time period (48 h). The results showed that: 1) OTUs (operational taxonomic units) in the treatment groups (31.4 +/- 0.55) exceeded those of the control groups (28.2 +/- 1.30) and the Shannon and Simpson indices were lower than the control groups (3.31 +/- 0.08 and 0.84 +/- 0.02, 3.45 +/- 0.09 and 0.88 +/- 0.01); 2) Gammaproteobacteria predominated in the treatment groups (44.71 +/- 2.13 %) while Alphaproteobacteria dominated in the controls (67.17 +/- 3.87 %); 3) Gammaproteobacteria and Alphaproteobacteria were important biomarkers in the treatment and control groups respectively (LDA > 4.0). PA improved the relative abundance of Alteromonas significantly and decreased the one of Rhodobacteraceae. PICRUSt analysis showed that the decrease of Rhodobacterceae was closely related with the decline of most functional genes in metabolism such as amino acid, carbohydrate, xenobiotics, cofactors and vitamins metabolism after PA-treated" |
| Keywords: | Allelopathy/*drug effects/genetics Bacteroidetes/drug effects/genetics/isolation & purification Dinoflagellida/*drug effects/genetics/growth & development Harmful Algal Bloom/*drug effects Hydroxybenzoates/*pharmacology Microbiota/*drug effects/genetics P; |
| Notes: | "MedlineChen, Qi Zhu, Bo Sun, Dong Liu, Weijie Sun, Xian Duan, Shunshan eng Netherlands 2020/08/28 Aquat Toxicol. 2020 Oct; 227:105591. doi: 10.1016/j.aquatox.2020.105591. Epub 2020 Aug 9" |
