| Title: | Phenylethylamine induces an increase in cytosolic Ca2+ in yeast |
| Author(s): | Pinontoan R; Krystofova S; Kawano T; Mori IC; Tsuji FI; Iida H; Muto S; |
| Address: | "Nagoya University Bioscience Center, Nagoya University, Japan" |
| Journal Title: | Biosci Biotechnol Biochem |
| ISSN/ISBN: | 0916-8451 (Print) 0916-8451 (Linking) |
| Abstract: | "Beta-phenylethylamine (PEA) induced an increase in cytosolic free calcium ion concentration ([Ca2+]c) in Saccharomyces cerevisiae cells monitored with transgenic aequorin, a Ca2+-dependent photoprotein. The PEA-induced [Ca2+]c increase was dependent on the concentrations of PEA applied, and the Ca2+ mostly originated from an extracellular source. Preceding the Ca2+ influx, H2O2 was generated in the cells by the addition of PEA. Externally added H2O2 also induced a [Ca2+]c increase. These results suggest that PEA induces the [Ca2+]c increase via H2O2 generation. The PEA-induced [Ca2+]c increase occurred in the mid1 mutant with a slightly smaller peak than in the wild-type strain, indicating that Mid1, a stretch-activated nonselective cation channel, may not be mainly involved in the PEA-induced Ca2+ influx. When PEA was applied, the MATa mid1 mutant was rescued from alpha-factor-induced death in a Ca2+-limited medium, suggesting that the PEA-induced [Ca2+]c increase can reinforce calcium signaling in the mating pheromone response pathway" |
| Keywords: | Calcium/*metabolism Cytosol/*drug effects/metabolism Mating Factor Peptides/metabolism Phenethylamines/*pharmacology Pheromones/metabolism Saccharomyces cerevisiae/*drug effects/metabolism; |
| Notes: | "MedlinePinontoan, Reinhard Krystofova, Svetlana Kawano, Tomonori Mori, Izumi C Tsuji, Frederick I Iida, Hidetoshi Muto, Shoshi eng Research Support, Non-U.S. Gov't England 2002/07/03 Biosci Biotechnol Biochem. 2002 May; 66(5):1069-74. doi: 10.1271/bbb.66.1069" |
