| Title: | "Smell of Infection: A Novel, Noninvasive Method for Detection of Fish Excretory-Secretory Proteins" |
| Author(s): | Pawluk RJ; Stuart R; Garcia de Leaniz C; Cable J; Morphew RM; Brophy PM; Consuegra S; |
| Address: | "College of Science, Biosciences , Swansea University , Swansea , SA2 8PP , U.K. Wales Veterinary Science Centre , Buarth, Aberystwyth , Ceredigion SY23 1ND , U.K. School of Biosciences , Cardiff University , Cardiff , CF10 3AX , U.K. IBERS , Aberystwyth University , Penglais, Aberystwyth , Ceredigion SY23 3FL , U.K" |
| DOI: | 10.1021/acs.jproteome.8b00953 |
| ISSN/ISBN: | 1535-3907 (Electronic) 1535-3893 (Print) 1535-3893 (Linking) |
| Abstract: | "Chemical signals are produced by aquatic organisms following predatory attacks or perturbations such as parasitic infection. Ectoparasites feeding on fish hosts are likely to cause release of similar alarm cues into the environment due to the stress, wounding, and immune response stimulated upon infection. Alarm cues are often released in the form of proteins, antimicrobial peptides, and immunoglobulins that provide important insights into bodily function and infection status. Here we outline a noninvasive method to identify potential chemical cues associated with infection in fish by extracting, purifying, and characterizing proteins from water samples from cultured fish. Gel free proteomic methods were deemed the most suitable for protein detection in saline water samples. It was confirmed that teleost proteins can be characterized from water and that variation in protein profiles could be detected between infected and uninfected individuals and fish and parasite only water samples. Our novel assay provides a noninvasive method for assessing the health condition of both wild and farmed aquatic organisms. Similar to environmental DNA monitoring methods, these proteomic techniques could provide an important tool in applied ecology and aquatic biology" |
| Keywords: | Animals Fish Diseases/*metabolism/parasitology Fish Proteins/*isolation & purification/metabolism Fishes/metabolism/*parasitology Pheromones/chemistry/metabolism Proteomics/*methods Water/metabolism/parasitology Kryptolebias marmoratus alarm cues gel free; |
| Notes: | "MedlinePawluk, Rebecca J Stuart, Rebekah Garcia de Leaniz, Carlos Cable, Joanne Morphew, Russell M Brophy, Peter M Consuegra, Sofia eng Research Support, Non-U.S. Gov't 2018/12/24 J Proteome Res. 2019 Mar 1; 18(3):1371-1379. doi: 10.1021/acs.jproteome.8b00953. Epub 2019 Jan 9" |
