| Title: | Orientation of Anopheles gambiae (Diptera: Culicidae) to Plant-Host Volatiles in a Novel Diffusion-Cage Olfactometer |
| Author(s): | Otienoburu PE; Nikbakhtzadeh MR; Foster WA; |
| Address: | "The Ohio State University, 318W 12th Ave., Columbus, Ohio 43210 (potienoburu@jcsu.edu; nik.nikbakht@gmail.com; foster.13@osu.edu), Johnson C. Smith University, 100 Beatties Ford Rd., Charlotte, NC 28216, and potienoburu@jcsu.edu. The Ohio State University, 318W 12th Ave., Columbus, Ohio 43210 (potienoburu@jcsu.edu; nik.nikbakht@gmail.com; foster.13@osu.edu), Department of Molecular & Cellular Biology, University of California Davis, 1 Shields Ave., Davis, CA 95616. The Ohio State University, 318W 12th Ave., Columbus, Ohio 43210 (potienoburu@jcsu.edu; nik.nikbakht@gmail.com; foster.13@osu.edu)" |
| ISSN/ISBN: | 0022-2585 (Print) 1938-2928 (Electronic) 0022-2585 (Linking) |
| Abstract: | "A novel diffusion-cage olfactometer tested the responses of Anopheles gambiae Giles to plant volatiles. Green-leaf volatiles are often released from cut or injured plant tissue and may alter the headspace of plants used in olfactometer assays. The diffusion-cage olfactometer is designed for use with whole, intact plants, hence giving a more realistic behavioral assay. Its simple plastic construction, ease of assembly, and accommodation to whole plants makes it a useful tool for measuring mosquito orientation to plant volatiles within large enclosures. We compared its performance to that of the more commonly used T-tube wind-tunnel olfactometer, by testing the orientation of mosquitoes to volatiles of a few prevalent plants of eastern Africa reportedly utilized by An. gambiae for sugar: Parthenium hysterophorus (Asteraceae), Ricinus communis (Euphorbiaceae), Lantana camara (Verbenaceae), and Senna occidentalis (Fabaceae). Results indicate that the diffusion-cage olfactometer is an effective alternative to conventional wind-tunnel olfactometers, to test mosquito orientation to plant volatiles under seminatural conditions" |
| Keywords: | Animals Anopheles/*physiology Magnoliopsida/chemistry Olfactometry/*instrumentation Volatile Organic Compounds Anopheles gambiae olfactometer plant attractant plant volatile screenhouse; |
| Notes: | "MedlineOtienoburu, Philip E Nikbakhtzadeh, Mahmood R Foster, Woodbridge A eng R01 AI077722/AI/NIAID NIH HHS/ R01-AI077722/AI/NIAID NIH HHS/ Evaluation Study Research Support, N.I.H., Extramural England 2015/10/28 J Med Entomol. 2016 Jan; 53(1):237-40. doi: 10.1093/jme/tjv162. Epub 2015 Oct 26" |
