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BMC Evol Biol

Title:		Transcriptomic response of female adult moths to host and non-host plants in two closely related species
Author(s):		Orsucci M; Audiot P; Nidelet S; Dorkeld F; Pommier A; Vabre M; Severac D; Rohmer M; Gschloessl B; Streiff R;
Address:		"CBGP, INRA, CIRAD, IRD, Montpellier SupAgro, Univ Montpellier, Montpellier, France. marion.orsucci@gmail.com. DGIMI, INRA, Univ Montpellier, Montpellier, France. marion.orsucci@gmail.com. Present address: Department of Ecology and Genetics, EBC, Uppsala University, Norbyvagen 18D, 75236, Uppsala, Sweden. marion.orsucci@gmail.com. CBGP, INRA, CIRAD, IRD, Montpellier SupAgro, Univ Montpellier, Montpellier, France. DIASCOPE, INRA, Mauguio, France. MGX-Montpellier GenomiX, c/o Institut de Genomique Fonctionnelle, 34094, Montpellier Cedex 5, France. CBGP, INRA, CIRAD, IRD, Montpellier SupAgro, Univ Montpellier, Montpellier, France. rejane.streiff@inra.fr. DGIMI, INRA, Univ Montpellier, Montpellier, France. rejane.streiff@inra.fr"
Journal Title:		BMC Evol Biol
Year:		2018
Volume:		20180920
Issue:		1
Page Number:		145 -
DOI:		10.1186/s12862-018-1257-3
ISSN/ISBN:		1471-2148 (Electronic) 1471-2148 (Linking)
Abstract:		"BACKGROUND: Divergent selection has been shown to promote speciation in many taxa and especially in phytophagous insects. In the Ostrinia species complex, the European corn borer (ECB) and adzuki bean borer (ABB) are two sibling species specialized to different host plants. The first is a well-known maize pest, whereas the second is a polyphagous species associated with various dicotyledons. Their specialization to host plants is driven by morphological, behavioral and physiological adaptations. In particular, previous studies have shown that ECB and ABB display marked behavior with regard to plant choice during oviposition, involving specific preference and avoidance mechanisms. In this study, our goal was to identify the mechanisms underlying this host-plant specialization in adult females through an analysis of their gene expression. We assembled and annotated a de novo reference transcriptome and measured differences in gene expression between ECB and ABB females, and between environments. We related differentially expressed genes to host preference behavior, and highlighted the functional categories involved. We also conducted a specific analysis of chemosensory genes, which are considered to be good candidates for host recognition before oviposition. RESULTS: We recorded more differentially expressed genes in ECB than in ABB samples, and noticed that the majority of genes potentially involved in the host preference were different between the two species. At the functional level, the response to plant environment in adult females involved many processes, including the metabolism of carbohydrates, lipids, proteins, and amino acids; detoxification mechanisms and immunity; and the chemosensory repertoire (as expected). Until now, most of the olfactory receptors described in Ostrinia spp. had been tested for their putative role in pheromone recognition by males. Here we observed that one specific olfactory receptor was clearly associated with ECB's discrimination between maize and mugwort conditions, highlighting a potential new candidate involved in plant odor discrimination in adult females. CONCLUSIONS: Our results are a first step toward the identification of candidate genes and functions involved in chemosensory processes, carbohydrate metabolism, and virus and retrovirus dynamics. These candidates provide new avenues for research into understanding the role of divergent selection between different environments in species diversification"
Keywords:		"Adaptation, Physiological/genetics Animals Female Gene Expression Profiling Linear Models Male Molecular Sequence Annotation Moths/*genetics Plants/*parasitology Principal Component Analysis Species Specificity Transcriptome/*genetics Adaptation Insect-pl;"
Notes:		"MedlineOrsucci, M Audiot, P Nidelet, S Dorkeld, F Pommier, A Vabre, M Severac, D Rohmer, M Gschloessl, B Streiff, R eng ANR-13-BSV7-0012-01/Agence Nationale de la Recherche (FR)/International PhD grant/Universite de Montpellier/International Research Support, Non-U.S. Gov't England 2018/09/22 BMC Evol Biol. 2018 Sep 20; 18(1):145. doi: 10.1186/s12862-018-1257-3"