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Saudi J Biol Sci

Title:		Production of a biopesticide on host and Non-Host serine protease inhibitors for red palm weevil in palm trees
Author(s):		Orfali R; Binsuwaileh A; Abu Al-Ala'a H; Bane-Gamea S; Zaidan N; Abdelazim M; Alhasan Ismael M; Perveen S; Majrashi N; Alluhayb K; Orfali RS;
Address:		"Department of Pharmacognosy, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi Arabia. Chair of Date Palm Research, Department of Plant Protection, College of Food and Agricultural Sciences, King Saud University, PO Box 2457, Riyadh 11451, Saudi Arabia. Chair of Protein Research, College of Science, King Saud University, PO Box 2457, Riyadh 11451, Saudi Arabia. National Center for Biotechnology, Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), P.O. Box 6086, Riyadh 11461, Saudi Arabia. Chapman University, School of Pharmacy, 9401 Jeronimo Rd, Irvine, CA 92618, USA"
Journal Title:		Saudi J Biol Sci
Year:		2020
Volume:		20200703
Issue:		10
Page Number:		2803 - 2808
DOI:		10.1016/j.sjbs.2020.06.048
ISSN/ISBN:		1319-562X (Print) 2213-7106 (Electronic) 2213-7106 (Linking)
Abstract:		"Serine proteases are essential metabolic enzymes in the midgut of many pests, including the red palm weevil (RPW), Rhynchophorus ferrugineus Olivier, which has a significant impact economically, environmentally and socially worldwide especially in the middle east. Some methods have been used to manage this pest such as trapping of RPW with pheromones, chemicals, and X-rays. However, these methods are costly, not effective and negatively impact the human. The main objective of this study is to contribute to the discovery of an eco-friendly pesticide to eradicate this infection by using serine protease inhibitors (SPIs) extracted from different parts of plant resources. In this research, both in vitro and in vivo effects of SPIs activity against RPW were examined. The protease inhibitors (PIs) activity was recorded in the crude extract that was isolated from the date's kernel (DKE), host and Calotropis latex (CLE), non-host. These PIs were partially purified by ammonium sulfate precipitation. The midgut tissue of RPW was extracted and analyzed for protases activity assay. PIs assays were consistent with the increased in the inhibitory activity against the midgut proteases after treatment with a DKE and CLE. The reduction of gut proteases by DKE solution and CLE was 39%, 18%, respectively. Partially purified DKE showed the most prominent inhibition pattern of protease activity of the gut extract. While, latex exhibited acute toxicity, imparting the least LC(50) (5.132 mg/mL) against RPW larvae. Taken together, these findings provide evidence for the hypothesis that SPIs activity may play an important role in enhancing the mortality of RPW and relieving the toxicity of insecticide in palm trees"
Keywords:		Calotropis latex Date's kernel Protease inhibitors Red palm weevil Rhynchophorus ferrugineus;
Notes:		"PubMed-not-MEDLINEOrfali, Raha Binsuwaileh, Arwa Abu Al-Ala'a, Hanan Bane-Gamea, Saja Zaidan, Nada Abdelazim, Mahmoud Alhasan Ismael, Mohamad Perveen, Shagufta Majrashi, Najwa Alluhayb, Khulud Orfali, Razan S eng Saudi Arabia 2020/10/01 Saudi J Biol Sci. 2020 Oct; 27(10):2803-2808. doi: 10.1016/j.sjbs.2020.06.048. Epub 2020 Jul 3"