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J Basic Microbiol

Title:		Characteristics of volatile organic compounds produced from five pathogenic bacteria by headspace-solid phase micro-extraction/gas chromatography-mass spectrometry
Author(s):		Chen J; Tang J; Shi H; Tang C; Zhang R;
Address:		"College of Life Science and Technology, Southwest University for Nationalities, Chengdu, China"
Journal Title:		J Basic Microbiol
Year:		2017
Volume:		20161122
Issue:		3
Page Number:		228 - 237
DOI:		10.1002/jobm.201600505
ISSN/ISBN:		1521-4028 (Electronic) 0233-111X (Linking)
Abstract:		"The characteristics of volatile compounds from five different bacterial species, Escherichia coli O157:H7, Salmonella Enteritidis, Shigella flexneri, Staphylococcus aureus, and Listeria monocytogenes, growing, respectively, in trypticase soy broth were monitored by headspace solid-phase micro-extraction/gas chromatography-mass spectrometry. The results showed that most volatile organic compounds (VOCs) of five pathogens started to increase after the sixth to tenth hour. Methyl ketones and long chain alcohols were representative volatiles for three Gram-negative bacteria. The especially high production of indole was characterized to E. coli O157:H7. The production of 3-hydroxy-2-butanone was indicative of the presence of two Gram-positive bacteria. Both 3-methyl-butanoic acid and 3-methyl-butanal were unique biomarkers for S. aureus. The population dynamics of individual pathogen could be monitored using the accumulation of VOCs correlated with its growth. And these five pathogens could be distinguishable though principle component analysis of 18 volatile metabolites. Moreover, the mixed culture of S. aureus and E. coli O157:H7 was also investigated. The levels of 3-methyl-butanal and 3-methyl-butanoic acid were largely reduced; while the level of indole almost unchanged and correlated with E. coli O157:H7 growth very well. The characteristics of volatiles from the five foodborne pathogens could lay a fundamental basis for further research into pathogen contamination control by detecting volatile signatures of pathogens"
Keywords:		Acetoin/metabolism Alcohols/chemistry Biomarkers/metabolism Culture Media/chemistry Escherichia coli O157/chemistry/growth & development/*metabolism Food Microbiology Gas Chromatography-Mass Spectrometry/methods Indoles/metabolism Ketones/chemistry Lister;
Notes:		"MedlineChen, Juan Tang, Junni Shi, Hui Tang, Cheng Zhang, Rong eng Germany 2016/11/23 J Basic Microbiol. 2017 Mar; 57(3):228-237. doi: 10.1002/jobm.201600505. Epub 2016 Nov 22"