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« Previous Abstract"Whole intact rapeseeds or sunflower oil in high-forage or high-concentrate diets affects milk yield, milk composition, and mammary gene expression profile in goats"    Next AbstractPrm1 targeting to contact sites enhances fusion during mating in Saccharomyces cerevisiae »

J Biol Chem


Title:Prm1 functions as a disulfide-linked complex in yeast mating
Author(s):Olmo VN; Grote E;
Address:"Department of Biochemistry and Molecular Biology, The Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland 21205, USA"
Journal Title:J Biol Chem
Year:2010
Volume:20091120
Issue:4
Page Number:2274 - 2283
DOI: 10.1074/jbc.M109.068874
ISSN/ISBN:1083-351X (Electronic) 0021-9258 (Print) 0021-9258 (Linking)
Abstract:"Prm1 is a pheromone-induced membrane glycoprotein that promotes plasma membrane fusion in yeast mating pairs. HA-Prm1 migrates at twice its expected molecular weight on non-reducing SDS-PAGE gels and coprecipitates with Prm1-TAP, indicating that Prm1 is a disulfide-linked homodimer. The N terminus of a plasma membrane-localized GFP-Prm1 endocytic mutant projects into the cytoplasm, where it is protected from low pH quenching in live cells and from external protease in spheroplasts. In a revised topological map, Prm1 has four transmembrane domains and two large extracellular loops. Mutation of all four cysteines in the extracellular loops blocked disulfide bond formation and destabilized the Prm1 homodimer without preventing Prm1 transport to contact sites in mating pairs. Cys(120) in loop 1 and Cys(545) in loop 2 form disulfide cross-links in the Prm1 homodimer and are required for fusion activity. Cys(120) lies between a hydrophobic segment formerly thought to be a transmembrane domain and an amphipathic helix. An interaction between either of these regions and the opposing membrane could promote fusion"
Keywords:Cell Membrane/enzymology Cysteine/genetics Dimerization Disulfides/*chemistry/*metabolism Endoplasmic Reticulum/enzymology Glycosylation Green Fluorescent Proteins/genetics Membrane Fusion/*physiology Membrane Proteins/*chemistry/genetics/*metabolism Muta;
Notes:"MedlineOlmo, Valerie N Grote, Eric eng T32 CA009110/CA/NCI NIH HHS/ Research Support, Non-U.S. Gov't 2009/11/26 J Biol Chem. 2010 Jan 22; 285(4):2274-83. doi: 10.1074/jbc.M109.068874. Epub 2009 Nov 20"

 
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