| Title: | The role of Cdc42 in signal transduction and mating of the budding yeast Saccharomyces cerevisiae |
| Address: | "Rockefeller University, New York, New York 10021, USA" |
| ISSN/ISBN: | 0021-9258 (Print) 0021-9258 (Linking) |
| Abstract: | "The small G-protein Cdc42 functions in many eukaryotic signal transduction pathways. In the budding yeast Saccharomyces cerevisiae, cells with defective Cdc42 fail to induce mating-specific genes in response to mating factor and to adopt the proper morphology for conjugation. Here we show that the failure of mating factor-induced transcription is largely the indirect result of arrest at a specific cell cycle position and/or the accumulation of high levels of the Cln1/2-Cdc28 kinase, a known repressor of mating factor signal transduction. Cdc42-defective cells with restored transcriptional induction have a partially restored mating ability but are still defective in the morphological response to mating factor. These results show that Cdc42 is not required for transduction of the mating factor signal per se but that it is essential for proper mating factor-induced morphogenesis" |
| Keywords: | "CDC28 Protein Kinase, S cerevisiae/biosynthesis Cell Cycle/drug effects/physiology Cell Cycle Proteins/*metabolism Crosses, Genetic Cyclins/biosynthesis Fungal Proteins/biosynthesis GTP Phosphohydrolases/metabolism GTP-Binding Proteins/*metabolism Genotyp;" |
| Notes: | "MedlineOehlen, L J Cross, F R eng GM49716/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. 1998/05/16 J Biol Chem. 1998 Apr 10; 273(15):8556-9. doi: 10.1074/jbc.273.15.8556" |
