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Plant Cell Physiol

Title:		Mucilage secretion regulated by sex pheromones in Closterium peracerosum-strigosum-littorale complex
Author(s):		Akatsuka S; Sekimoto H; Iwai H; Fukumoto RH; Fujii T;
Address:		"Graduate School of Life Sciences, Toyo University, 1-1-1 Izumino, Itakura-machi, Ora-gun, Gunma, 374-0193 Japan"
Journal Title:		Plant Cell Physiol
Year:		2003
Volume:		44
Issue:		10
Page Number:		1081 - 1087
DOI:		10.1093/pcp/pcg131
ISSN/ISBN:		0032-0781 (Print) 0032-0781 (Linking)
Abstract:		"When mating-type plus (mt+) and minus (mt-) cells of the Closterium peracerosum-strigosum-littorale complex were mixed in nitrogen-depleted mating medium, secretion of mucilage containing uronic acid from cells was markedly activated and the mucilage accumulated around the cells. Substances with the ability to stimulate mucilage secretion from mt+ and mt- cells were detected in media in which mt- and mt+ cells had been separately cultured, respectively. We designated the active substances secreted from mt+ and mt- cells mucilage secretion-stimulating pheromone (MS-SP)-plus and MS-SP-minus, respectively. Activity of MS-SP-plus and MS-SP-minus decreased to 20% level by incubation at 80 degrees C for 10 min. Light was indispensable for the secretion of mucilage. The secretion of MS-SP-plus and MS-SP-minus decreased with dark treatment. MS-SP-plus eluted at around 95 k from a gel filtration column, and reacted with antibodies against two subunits of protoplast-release-inducing protein (PR-IP), which induces protoplast release from mt- cells. MS-SP-minus eluted at around 20 k from a gel filtration column, and reacted with an antibody against the PR-IP inducer, which induces the secretion of PR-IP from mt+ cells. In addition, purified PR-IP and PR-IP inducer stimulated mucilage secretion from mt- and mt+ cells, respectively. These results strongly suggested that MS-SP-plus and MS-SP-minus were the same molecules as the PR-IP and the PR-IP inducer, respectively"
Keywords:		Adhesives/chemistry/*metabolism Clostridium/*physiology Kinetics Magnoliopsida/*physiology Plant Growth Regulators/*physiology Uronic Acids/analysis;
Notes:		"MedlineAkatsuka, Satoko Sekimoto, Hiroyuki Iwai, Hiroaki Fukumoto, Ryo-hei Fujii, Tadashi eng Research Support, Non-U.S. Gov't Japan 2003/10/29 Plant Cell Physiol. 2003 Oct; 44(10):1081-7. doi: 10.1093/pcp/pcg131"