| Title: | Profiling of volatile terpenes from almond (Prunus dulcis) young fruits and characterization of seven terpene synthase genes |
| Author(s): | Nawade B; Yahyaa M; Reuveny H; Shaltiel-Harpaz L; Eisenbach O; Faigenboim A; Bar-Yaakov I; Holland D; Ibdah M; |
| Address: | "Newe Yaar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay, 30095, Israel. Migal Galilee Research Institute, P.O. Box 831, Kiryat Shmona, 11016, Israel. Migal Galilee Research Institute, P.O. Box 831, Kiryat Shmona, 11016, Israel; Tel Hai college, Upper Galilee, 12210, Israel. Institute of Plant Science, The Volcani Center, ARO, P.O. Box 6, Bet Dagan, 50250, Israel. Newe Yaar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay, 30095, Israel. Electronic address: mwafaq@volcani.agri.gov.il" |
| DOI: | 10.1016/j.plantsci.2019.110187 |
| ISSN/ISBN: | 1873-2259 (Electronic) 0168-9452 (Linking) |
| Abstract: | "Almond (Prunus dulcis) is an agricultural and economically important fruit tree from the Rosaceae family used in the food industry. The monoterpenes and sesquiterpenes perform important ecological functions such as insecticidal and antifeedant activities against various insects. The young fruits of the different almond varieties were found to produce considerable amounts of terpene volatiles, including linalool and geraniol. To identify terpene synthases (TPSs) involved in the production of these volatile terpenes, existing genome databases of the Rosaceae were screened for almond genes with significant sequence similarity to other plants TPSs. Bioinformatics analysis led to the identification of seven putative TPSs genes with complete open reading frames. We characterized the enzymes encoded by these seven complementary DNAs: the monoterpene synthases PdTPS1, PdTPS3, PdTPS5, and PdTPS6 belong to the TPS-b clade, which catalyzes the formation of beta-phellandrene, geraniol, linalool, and farnesene, respectively. The sesquiterpene synthases PdTPS2 and PdTPS4, which belong to the TPS-a clade mainly catalyze the formation of bergamotene, while another sesquiterpene synthase, PdTPS7, from the TPS-g clade showed nerolidol synthase activity. The qRT-PCR analysis revealed that the various tissues of almond varieties showed differential transcription for all these PdTPSs genes" |
| Keywords: | Acyclic Monoterpenes/metabolism Alkyl and Aryl Transferases/genetics/*metabolism Computational Biology Cyclohexane Monoterpenes/metabolism Fruit/enzymology/genetics Phylogeny Plant Proteins/genetics/metabolism Prunus dulcis/*enzymology/genetics Terpenes/*; |
| Notes: | "MedlineNawade, Bhagwat Yahyaa, Mosaab Reuveny, Haim Shaltiel-Harpaz, Liora Eisenbach, Ori Faigenboim, Adi Bar-Yaakov, Irit Holland, Doron Ibdah, Mwafaq eng Ireland 2019/09/05 Plant Sci. 2019 Oct; 287:110187. doi: 10.1016/j.plantsci.2019.110187. Epub 2019 Jul 9" |
