« Previous AbstractSerotonin modulates worker responsiveness to trail pheromone in the ant Pheidole dentata    Next AbstractWhy does Staphylococcus aureus secrete an Enterococcus faecalis-specific pheromone? »

J Biotechnol

Title:		"Synthesis of alpha-hydroxy ketones and vicinal diols with the Bacillus licheniformis DSM 13(T) butane-2,3-diol dehydrogenase"
Author(s):		Muschallik L; Kipp CR; Recker I; Bongaerts J; Pohl M; Gellissen M; Schoning MJ; Selmer T; Siegert P;
Address:		"Institute of Nano- and Biotechnologies, Aachen University of Applied Sciences, 52428, Julich, Germany. IBG-1: Biotechnology, Forschungszentrum Julich, 52425, Julich, Germany. Institute of Nano- and Biotechnologies, Aachen University of Applied Sciences, 52428, Julich, Germany. Electronic address: siegert@fh-aachen.de"
Journal Title:		J Biotechnol
Year:		2020
Volume:		20200922
Issue:
Page Number:		61 - 70
DOI:		10.1016/j.jbiotec.2020.09.016
ISSN/ISBN:		1873-4863 (Electronic) 0168-1656 (Linking)
Abstract:		"The enantioselective synthesis of alpha-hydroxy ketones and vicinal diols is an intriguing field because of the broad applicability of these molecules. Although, butandiol dehydrogenases are known to play a key role in the production of 2,3-butandiol, their potential as biocatalysts is still not well studied. Here, we investigate the biocatalytic properties of the meso-butanediol dehydrogenase from Bacillus licheniformis DSM 13(T) (BlBDH). The encoding gene was cloned with an N-terminal StrepII-tag and recombinantly overexpressed in E. coli. BlBDH is highly active towards several non-physiological diketones and alpha-hydroxyketones with varying aliphatic chain lengths or even containing phenyl moieties. By adjusting the reaction parameters in biotransformations the formation of either the alpha-hydroxyketone intermediate or the diol can be controlled"
Keywords:		"*Bacillus licheniformis/genetics Butylene Glycols Escherichia coli/genetics *Ketones Acetoin reductase/butane-2, 3-diol dehydrogenase Bacillus licheniformis Biocatalysis Meso-butane-2, 3-diol dehydrogenase SDR Pheromones;"
Notes:		"MedlineMuschallik, Lukas Kipp, Carina Ronja Recker, Inga Bongaerts, Johannes Pohl, Martina Gellissen, Melanie Schoning, Michael J Selmer, Thorsten Siegert, Petra eng Netherlands 2020/09/26 J Biotechnol. 2020 Dec 20; 324:61-70. doi: 10.1016/j.jbiotec.2020.09.016. Epub 2020 Sep 22"