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Invert Neurosci

Title:		Species-specific pheromonal compounds induce distinct conformational changes of pheromone binding protein subtypes from Antheraea polyphemus
Author(s):		Mohl C; Breer H; Krieger J;
Address:		"University of Hohenheim, Institute of Physiology, Garbenstrasse 30, 70599 Stuttgart, Germany"
Journal Title:		Invert Neurosci
Year:		2002
Volume:		20020713
Issue:		4
Page Number:		165 - 174
DOI:		10.1007/s10158-002-0018-5
ISSN/ISBN:		1354-2516 (Print) 1354-2516 (Linking)
Abstract:		"We have investigated the structural features of three pheromone binding protein (PBP) subtypes from Antheraea polyphemus and monitored possible changes induced upon interaction with the Antheraea pheromonal compounds 4E,9Z-14:Ac [(E4,Z9)-tetradecadienyl-1-acetate], 6E,11Z-16:Ac [(E6,Z11)-hexadecadienyl-1-acetate], and 6E,11Z-16:Al [(E6,Z11)-hexadecadienal]. Circular dichroism and second derivative UV-difference spectroscopy data demonstrate that the structure of subtype PBP1 significantly changes upon binding of 4E,9Z-14:Ac. The related 6E,11Z-16:Ac was less effective and 6E,11Z-16:Al showed only a small effect. In contrast, in subtype PBP2 pronounced structural changes were only induced by the 6E,11Z-16:Al, and the subtype PBP3 did not show any considerable changes in response to the pheromonal compounds. The UV-spectroscopic data suggest that histidine residues are likely to be involved in the ligand-induced structural changes of the proteins, and this notion was confirmed by site-directed mutagenesis experiments. These results demonstrate that appropriate ligands induce structural changes in PBPs and provide evidence for ligand specificity of these proteins"
Keywords:		Animals Carrier Proteins/*chemistry/*drug effects Circular Dichroism Insect Hormones/chemistry Insect Proteins/*chemistry/*drug effects Intercellular Signaling Peptides and Proteins Moths Pheromones/*pharmacology Protein Conformation/drug effects Recombin;
Notes:		"MedlineMohl, Claudia Breer, Heinz Krieger, Jurgen eng Research Support, Non-U.S. Gov't Germany 2002/12/19 Invert Neurosci. 2002 Oct; 4(4):165-74. doi: 10.1007/s10158-002-0018-5. Epub 2002 Jul 13"