| Title: | A period of 10 weeks of increased protein consumption does not alter faecal microbiota or volatile metabolites in healthy older men: a randomised controlled trial |
| Author(s): | Mitchell SM; McKenzie EJ; Mitchell CJ; Milan AM; Zeng N; D'Souza RF; Ramzan F; Sharma P; Rettedal E; Knowles SO; Roy NC; Sjodin A; Wagner KH; O'Sullivan JM; Cameron-Smith D; |
| Address: | "Liggins Institute, University of Auckland, Auckland, New Zealand. Riddet Institute, Massey University, Palmerston North, New Zealand. Department of Physiology, University of Auckland, Auckland, New Zealand. School of Kinesiology, University of British Columbia, Vancouver, Canada. Food Nutrition & Health Team, AgResearch, Palmerston North, New Zealand. The High-Value Nutrition National Science Challenge, Auckland, New Zealand. Discipline of Nutrition, School of Medical Sciences, University of Auckland, Auckland, New Zealand. Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland, Auckland, New Zealand. Department of Nutrition, Exercise and Sports, Copenhagen University, Copenhagen, Denmark. Department of Nutritional Sciences and Research Platform Active Ageing, University of Vienna, Vienna, Austria. Singapore Institute for Clinical Sciences, Agency for Science, Technology, and Research, Singapore" |
| ISSN/ISBN: | 2048-6790 (Electronic) 2048-6790 (Linking) |
| Abstract: | "Diet has a major influence on the composition and metabolic output of the gut microbiome. Higher-protein diets are often recommended for older consumers; however, the effect of high-protein diets on the gut microbiota and faecal volatile organic compounds (VOC) of elderly participants is unknown. The purpose of the study was to establish if the faecal microbiota composition and VOC in older men are different after a diet containing the recommended dietary intake (RDA) of protein compared with a diet containing twice the RDA (2RDA). Healthy males (74?na2 (sd 3?na6) years; n 28) were randomised to consume the RDA of protein (0?na8 g protein/kg body weight per d) or 2RDA, for 10 weeks. Dietary protein was provided via whole foods rather than supplementation or fortification. The diets were matched for dietary fibre from fruit and vegetables. Faecal samples were collected pre- and post-intervention for microbiota profiling by 16S ribosomal RNA amplicon sequencing and VOC analysis by head space/solid-phase microextraction/GC-MS. After correcting for multiple comparisons, no significant differences in the abundance of faecal microbiota or VOC associated with protein fermentation were evident between the RDA and 2RDA diets. Therefore, in the present study, a twofold difference in dietary protein intake did not alter gut microbiota or VOC indicative of altered protein fermentation" |
| Keywords: | "Aged *Diet, High-Protein *Dietary Proteins Feces/chemistry/microbiology Gastrointestinal Microbiome Humans Male Microbiota/*drug effects Nutritional Requirements Treatment Outcome Volatile Organic Compounds/analysis 16S amplicon sequencing 16S rRNA, 16S r;" |
| Notes: | "MedlineMitchell, S M McKenzie, E J Mitchell, C J Milan, A M Zeng, N D'Souza, R F Ramzan, F Sharma, P Rettedal, E Knowles, S O Roy, N C Sjodin, A Wagner, K-H O'Sullivan, J M Cameron-Smith, D eng Randomized Controlled Trial Research Support, Non-U.S. Gov't England 2020/08/04 J Nutr Sci. 2020 Jul 3; 9:e25. doi: 10.1017/jns.2020.15. eCollection 2020" |
