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Plant J

Title:		WRKY40 and WRKY6 act downstream of the green leaf volatile E-2-hexenal in Arabidopsis
Author(s):		Mirabella R; Rauwerda H; Allmann S; Scala A; Spyropoulou EA; de Vries M; Boersma MR; Breit TM; Haring MA; Schuurink RC;
Address:		"Department of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, Amsterdam, 1098 XH, The Netherlands. MAD, Dutch Genomics Service & Support Provider, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, Amsterdam, 1098 XH, The Netherlands"
Journal Title:		Plant J
Year:		2015
Volume:		83
Issue:		6
Page Number:		1082 - 1096
DOI:		10.1111/tpj.12953
ISSN/ISBN:		1365-313X (Electronic) 0960-7412 (Linking)
Abstract:		"Plants are known to be responsive to volatiles, but knowledge about the molecular players involved in transducing their perception remains scarce. We study the response of Arabidopsis thaliana to E-2-hexenal, one of the green leaf volatiles (GLV) that is produced upon wounding, herbivory or infection with pathogens. We have taken a transcriptomics approach to identify genes that are induced by E-2-hexenal, but not by defence hormones or other GLVs. Furthermore, by studying the promoters of early E-2-hexenal-induced genes we determined that the only statistically enriched cis-element was the W-box motif. Since members of the plant-specific family of WRKY transcription factors act in trans on this cis-element, we focused on WRKY6, 40 and 53 that were most strongly induced by E-2-hexenal. Root elongation of Arabidopsis seedlings of the wrky40 wrky6 double mutant was much less inhibited than in wt plants, similar to the E-2-hexenal-responsive mutant her1, which is perturbed in gamma-amino butyric acid (GABA) metabolism. The induction of several of the E-2-hexenal-specific genes was much higher in the wrky40, wrky6 or wrky40 wrky6 mutants, including GAD4, a glutamate decarboxylase that catalyzes the formation of GABA from glutamate. In conclusion, WRKY6 and 40 seem to act as important players transducing E-2-hexenal perception"
Keywords:		"Aldehydes/*metabolism/pharmacology Arabidopsis/drug effects/genetics/*metabolism Arabidopsis Proteins/genetics/*metabolism Gene Expression Profiling Gene Expression Regulation, Plant/drug effects Mutation Plant Leaves/genetics/*metabolism Plant Roots/gene;"
Notes:		"MedlineMirabella, Rossana Rauwerda, Han Allmann, Silke Scala, Alessandra Spyropoulou, Eleni A de Vries, Michel Boersma, Maaike R Breit, Timo M Haring, Michel A Schuurink, Robert C eng Research Support, Non-U.S. Gov't England 2015/08/06 Plant J. 2015 Sep; 83(6):1082-96. doi: 10.1111/tpj.12953"