| Title: | Profiling of VOCs released from different salivary bacteria treated with non-lethal concentrations of silver nitrate |
| Author(s): | Milanowski M; Monedeiro F; Zloch M; Ratiu IA; Pomastowski P; Ligor T; De Martinis BS; Buszewski B; |
| Address: | "Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarina Str, 87-100, Torun, Poland; Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University, 4 Wilenska Str, 87-100, Torun, Poland. Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarina Str, 87-100, Torun, Poland; Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University, 4 Wilenska Str, 87-100, Torun, Poland; Department of Chemistry, Faculty of Philosophy, Science and Letters of Ribeirao Preto, University of Sao Paulo, CEP 14040-901, Ribeirao Preto, Brazil. Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarina Str, 87-100, Torun, Poland; Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University, 4 Wilenska Str, 87-100, Torun, Poland; (d)Babes-Bolyai University, Faculty of Chemistry and Chemical Engineering, 11 Arany Janos, RO-400028, Cluj-Napoca, Romania. Department of Chemistry, Faculty of Philosophy, Science and Letters of Ribeirao Preto, University of Sao Paulo, CEP 14040-901, Ribeirao Preto, Brazil. Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarina Str, 87-100, Torun, Poland; Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University, 4 Wilenska Str, 87-100, Torun, Poland. Electronic address: bbusz@chem.uni.torun.pl" |
| ISSN/ISBN: | 1096-0309 (Electronic) 0003-2697 (Linking) |
| Abstract: | "Considering the shortcomings related to antibiotics usage, the introduction of other bacteriostatic and bactericidal agents that present synergetic effects or standalone properties is urgently needed. AgNO(3) is an important bactericidal agent, which imparts various functions on bacteria dependent on its concentration. Therefore, an understanding of its mechanisms of action in infinitesimal concentrations plays an important role which can ultimately lead to AgNO(3) involvement in the pharmaceutical industry. The monitoring of VOC (volatile organic compound) profiles emitted by bacteria is a simple method to assess changes occurring in bacterial metabolism. In this study, VOCs of Hafnia alvei, Pseudomonas luteola and Staphylococcus warneri cultures were analyzed both in the absence and in the presence of three concentrations of AgNO(3). Headspace solid-phase microextraction gas chromatography/mass spectrometry (HS-SPME-GC/MS) was employed for extraction and analysis. After supplementation with AgNO(3), changes in the emitted fingerprints were investigated. Odorants associated with mouth-related and systemic diseases, like dimethyl trisulfide, indole (halitosis) and 2-hexanone (celiac disease), were also affected by addition of AgNO(3). Statistical tests proved discrimination between obtained profiles with more that 90% variability. Moreover, physiological states of bacteria after dosage with various concentration of stressing agent were investigated and explained by the mechanisms of action" |
| Keywords: | Hafnia alvei/*drug effects Humans Pseudomonas/*drug effects Saliva/*microbiology Silver Nitrate/*pharmacology Staphylococcus/*drug effects Volatile Organic Compounds/*metabolism AgNO(3) effect Hs-spme-gc/ms Salivary bacteria VOCs profiles; |
| Notes: | "MedlineMilanowski, Maciej Monedeiro, Fernanda Zloch, Michal Ratiu, Ileana-Andreea Pomastowski, Pawel Ligor, Tomasz De Martinis, Bruno S Buszewski, Boguslaw eng Research Support, Non-U.S. Gov't 2019/05/16 Anal Biochem. 2019 Aug 1; 578:36-44. doi: 10.1016/j.ab.2019.05.007. Epub 2019 May 11" |
