| Title: | Subtle selectivity in a pheromone sensor triumvirate desynchronizes competence and predation in a human gut commensal |
| Author(s): | Mignolet J; Cerckel G; Damoczi J; Ledesma-Garcia L; Sass A; Coenye T; Nessler S; Hols P; |
| Address: | "Biochemistry and Genetics of Microorganisms (BGM), Louvain Institute of Biomolecular Science and Technology, Universite catholique de Louvain, Louvain-la-Neuve, Belgium. Syngulon, Seraing, Belgium. Laboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium. Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Universite Paris-Saclay, 91198, Gif-sur-Yvette cedex, France" |
| ISSN/ISBN: | 2050-084X (Electronic) 2050-084X (Linking) |
| Abstract: | "Constantly surrounded by kin or alien organisms in nature, eukaryotes and prokaryotes developed various communication systems to coordinate adaptive multi-entity behavior. In complex and overcrowded environments, they require to discriminate relevant signals in a myriad of pheromones to execute appropriate responses. In the human gut commensal Streptococcus salivarius, the cytoplasmic Rgg/RNPP regulator ComR couples competence to bacteriocin-mediated predation. Here, we describe a paralogous sensor duo, ScuR and SarF, which circumvents ComR in order to disconnect these two physiological processes. We highlighted the recurring role of Rgg/RNPP in the production of antimicrobials and designed a robust genetic screen to unveil potent/optimized peptide pheromones. Further mutational and biochemical analyses dissected the modifiable selectivity toward their pheromone and operating sequences at the subtle molecular level. Additionally, our results highlight how we might mobilize antimicrobial molecules while silencing competence in endogenous populations of human microflora and temper gut disorders provoked by bacterial pathogens" |
| Keywords: | "Anti-Bacterial Agents/metabolism Bacterial Proteins/*metabolism Bacteriocins/*metabolism DNA Transformation Competence/*drug effects *Gastrointestinal Microbiome Gene Expression Regulation, Bacterial/drug effects Gene Regulatory Networks/drug effects Huma;" |
| Notes: | "MedlineMignolet, Johann Cerckel, Guillaume Damoczi, Julien Ledesma-Garcia, Laura Sass, Andrea Coenye, Tom Nessler, Sylvie Hols, Pascal eng PDR T.0110.18-F/Fonds De La Recherche Scientifique - FNRS/International Project P7/28/BELSPO/International RPR1620203/Walloon Region (Belgium)/International Research Support, Non-U.S. Gov't England 2019/08/23 Elife. 2019 Aug 21; 8:e47139. doi: 10.7554/eLife.47139" |
