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Methods Enzymol

Title:		Ultra-high performance liquid chromatography-mass spectrometry analysis of plant apocarotenoids
Author(s):		Mi J; Moreno JC; Alagoz Y; Liew KX; Balakrishna A; Zheng X; Al-Babili S;
Address:		"The BioActives Lab, Center for Desert Agriculture, Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia. The BioActives Lab, Center for Desert Agriculture, Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia. Electronic address: salim.babili@kaust.edu.sa"
Journal Title:		Methods Enzymol
Year:		2022
Volume:		20211104
Issue:
Page Number:		285 - 309
DOI:		10.1016/bs.mie.2021.10.012
ISSN/ISBN:		1557-7988 (Electronic) 0076-6879 (Linking)
Abstract:		"Apocarotenoids (APOs) are a class of carotenoid oxidation products with high structural and functional diversity. Apart from serving as precursors of phytohormones, fungal pheromones and vitamin A, several APOs act as signaling molecules involved in stress response and growth as regulators in plants. To comprehensively profile plant APOs, we established an improved ultra-high performance liquid chromatography-hybrid quadrupole-Orbitrap mass spectrometer (UHPLC-Q-Orbitrap MS) analytical platform. The improved APO analytical platform consists of an optimized sequential APO sample preparation and multiple UHPLC-MS detection methods and was successfully used to identify and quantify multiple subclasses of APOs from tomato fruits. By integrating ultrasound-assisted extraction, solid phase extraction, and chemical derivatization, the improved sequential APOs sample preparation facilitates the simultaneous preparation of different subclasses of APOs from plant materials. In addition, multiple UHPLC-MS detection methods enables high throughput analysis of APOs. Application of this analytical strategy can make important contributions to understanding the function of these compounds and significantly facilitate the elucidation of plant APO metabolism"
Keywords:		"Carotenoids Chromatography, High Pressure Liquid/methods Mass Spectrometry/methods *Plants *Solid Phase Extraction/methods Apocarotenoids Diapocarotenals Glycosylated apocarotenoids Tomato Ultra-high performance liquid chromatography-mass spectrometry;"
Notes:		"MedlineMi, Jianing Moreno, Juan C Alagoz, Yagiz Liew, Kit Xi Balakrishna, Aparna Zheng, Xiongjie Al-Babili, Salim eng Research Support, Non-U.S. Gov't 2022/07/26 Methods Enzymol. 2022; 670:285-309. doi: 10.1016/bs.mie.2021.10.012. Epub 2021 Nov 4"