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J Ind Microbiol Biotechnol

Title:		Rapid identification of microbial VOCs from tobacco molds using closed-loop stripping and gas chromatography/time-of-flight mass spectrometry
Author(s):		Meruva NK; Penn JM; Farthing DE;
Address:		"Philip Morris USA Postgraduate Research Program, P.O. Box 26583, Richmond, VA 23261, USA"
Journal Title:		J Ind Microbiol Biotechnol
Year:		2004
Volume:		20041026
Issue:		10
Page Number:		482 - 488
DOI:		10.1007/s10295-004-0175-0
ISSN/ISBN:		1367-5435 (Print) 1367-5435 (Linking)
Abstract:		"Several microbial volatile organic compounds (MVOCs) that can serve as potential chemical markers for microbial contamination in tobacco have been identified. Four different fungal species, Aspergillus niger (AN), A. ornatus (AO), Pencillium chrysogenum (PC) and Rhizopus stolonifer (RS), commonly reported in moldy tobacco were cultured and screened for MVOCs. Because the MVOCs emitted by a microbial species are substrate specific, the fungal strains were separately grown on potato dextrose agar (PDA) and tobacco products. MVOCs from the mold cultures grown on PDA and tobacco products were extracted using closed-loop stripping analysis (CLSA) and identified by gas chromatography/time-of-flight mass spectrometry (GC/TOF-MS). Some of the prominent tobacco mold markers identified by this method include: 1-octen-3-ol; 2-octen-1-ol; 2-methyl-1-butanol; 3-methyl-1-butanol; 1-octene and 2-pentanone. In particular, 1-octen-3-ol was detected in all the mold cultures and moldy tobacco samples analyzed. Olfactory evaluation of 1-octen-3-ol indicated a characteristic musty odor and the odor threshold was determined to be approximately 200 ng/ml. The limits of detection for 1-octen-3-ol using GC/TOF-MS and GC/mass selective detector (MSD) in the full-scan mode and selected ion monitoring (SIM) mode were investigated. The CLSA-GC/TOF-MS demonstrates a fast, sensitive and semi-quantitative analytical technique for screening tobacco materials for the presence of mold via chemical markers of microbial contamination"
Keywords:		Aspergillus/chemistry/growth & development Aspergillus niger/chemistry/growth & development Fungi/*chemistry/*growth & development Gas Chromatography-Mass Spectrometry/methods Organic Chemicals/*analysis Penicillium chrysogenum/chemistry/growth & developm;
Notes:		"MedlineMeruva, N K Penn, J M Farthing, D E eng Evaluation Study Germany 2004/11/02 J Ind Microbiol Biotechnol. 2004 Nov; 31(10):482-8. doi: 10.1007/s10295-004-0175-0. Epub 2004 Oct 26"