| Title: | A proteomic analysis of Helicoverpa armigera adults after exposure to UV light irradiation |
| Author(s): | Meng JY; Zhang CY; Lei CL; |
| Address: | "Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, Institute of Insect Resources, Huazhong Agricultural University, Wuhan 430070, China" |
| DOI: | 10.1016/j.jinsphys.2009.11.015 |
| ISSN/ISBN: | 1879-1611 (Electronic) 0022-1910 (Linking) |
| Abstract: | "Ultraviolet (UV) light (blacklight), which emits UV in the range of 320-400nm, has been used worldwide in light trapping of insect pests. To gain a better understanding of the response of Helicoverpa armigera adults to UV light irradiation, we carried out a comparative proteomic analysis. Three-day-old adults were exposed to UV light for 1h. Total proteins were extracted and separated by two-dimensional gel electrophoresis. More than 1200 protein spots were reproducibly detected, including 12 that were more abundant and 21 less abundant. Mass spectrometry analysis and database searching helped us to identify 29 differentially abundant proteins. The identified proteins were categorized into several functional groups including signal transduction, RNA processing, protein processing, stress response, metabolisms, and cytoskeleton structure, etc. This study is the first analysis of differentially expressed proteins in phototactic insects under UV light irradiation conditions and gives new insights into the adaptation mechanisms responsive to UV light irradiation stress" |
| Keywords: | "Animals Electrophoresis, Gel, Two-Dimensional Female Insect Proteins/chemistry/genetics/metabolism Male Molecular Sequence Data Moths/*chemistry/genetics/metabolism/*radiation effects *Proteomics Ultraviolet Rays;" |
| Notes: | "MedlineMeng, Jian-Yu Zhang, Chang-Yu Lei, Chao-Liang eng Research Support, Non-U.S. Gov't England 2009/12/01 J Insect Physiol. 2010 Apr; 56(4):405-11. doi: 10.1016/j.jinsphys.2009.11.015. Epub 2009 Dec 4" |
