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Int J Anal Chem

Title:		Preparative Separation of Three Monoterpenes from Perilla frutescens var. crispa Using Centrifugal Partition Chromatography
Author(s):		Nam B; Paudel SB; Kim JB; Jin CH; Lee D; Nam JW; Han AR;
Address:		"Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup-si, Jeollabuk-do 56212, Republic of Korea. College of Pharmacy, Yeungnam University, Gyeongsan-si, Gyeongsangbuk-do 38541, Republic of Korea. Department of Biosystems and Biotechnology, Korea University, Seoul 02841, Republic of Korea"
Journal Title:		Int J Anal Chem
Year:		2019
Volume:		20190109
Issue:
Page Number:		8751345 -
DOI:		10.1155/2019/8751345
ISSN/ISBN:		1687-8760 (Print) 1687-8779 (Electronic) 1687-8760 (Linking)
Abstract:		"Three monoterpenes, namely, 9-hydroxy isoegomaketone (1), isoegomaketone (2), and perilla ketone (3), were successfully separated from the supercritical carbon dioxide (SC-CO(2)) extract of the leaves of Perilla frutescens var. crispa (cv. Antisperill; Lamiaceae) by centrifugal partition chromatography (CPC). To obtain large quantities of these materials required for studies on their mechanism of action and in vivo effectiveness in inflammation, we used CPC because of its high loading capacity and reproducibility to purify the three compounds. Compound 1 (2.60 mg, 96.7% purity at 254 nm) was purified from 500 mg of the SC-CO(2) extract of P. frutescens var. crispa (cv. Antisperill), using a two-phase solvent system comprising n-hexane/ethyl acetate/ethanol/water (5:5:5:5 v/v) in a descending mode. As compounds 2 (56.1 mg, 97.6% purity at 254 nm) and 3 (78.6 mg, 96.1% purity at 254 nm) are highly volatile and difficult to recover from an aqueous mobile phase after purification during the drying process, they were obtained from the same amount of the processed extract in an ascending mode using the upper organic phase as the mobile phase (n-hexane/ethyl acetate/ethanol/water, 8:2:8:2 v/v). The structures of compounds 1-3 were confirmed by (1)H- and (13)C-NMR analysis. Thus, based on our findings, we recommend centrifugal partition chromatography as a powerful technique for purifying the active principal compounds 1 and 2 from the leaves of P. frutescens var. crispa"
Keywords:
Notes:		"PubMed-not-MEDLINENam, Bomi Paudel, Sunil Babu Kim, Jin-Baek Jin, Chang Hyun Lee, Dongho Nam, Joo-Won Han, Ah-Reum eng Egypt 2019/02/08 Int J Anal Chem. 2019 Jan 9; 2019:8751345. doi: 10.1155/2019/8751345. eCollection 2019"